Fgf17 RT-PCR Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:200761 Kasberg AD, et al., SP8 regulates signaling centers during craniofacial development. Dev Biol. 2013 Sep 15;381(2):312-23
Assay type:	RT-PCR
MGI Accession ID:	MGI:7354995
Gene symbol:	Fgf17
Gene name:	fibroblast growth factor 17

Probe:	Fgf17-pK, Fgf17-pL
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Actb or Tubb5.

Results

Image: 5G Fgf17 ©

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
E8.5 WT	E8.5	TS13: anterior neural ridge TS13: facial neural crest TS13: neural fold	Present	Not Specified; total RNA	Not Specified		Not Specified	cranial neural folds
E8.5 Sp8-/-	E8.5	TS13: anterior neural ridge TS13: facial neural crest TS13: neural fold	Present (a)	Not Specified; total RNA	involves: 129S1/Sv * 129X1/SvJ	Sp8^tm1Smb/Sp8^tm1Smb	Not Specified	cranial neural folds
E9.5 WT	E9.5	TS15: face TS15: neural plate	Present	Not Specified; total RNA	Not Specified		Not Specified	middle to anterior facial structures including the anterior neural ridge
E9.5 Sp8-/-	E9.5	TS15: face TS15: neural plate	Present (a)	Not Specified; total RNA	involves: 129S1/Sv * 129X1/SvJ	Sp8^tm1Smb/Sp8^tm1Smb	Not Specified	middle to anterior facial structures including the anterior neural ridge
E10.5 WT	E10.5	TS17: latero-nasal process TS17: medial-nasal process TS17: nasal pit	Present	Not Specified; total RNA	Not Specified		Not Specified	olfactory pit, lateral nasal prominences, medial nasal prominences
E10.5 Sp8-/-	E10.5	TS17: latero-nasal process TS17: medial-nasal process TS17: nasal pit	Present (a)	Not Specified; total RNA	involves: 129S1/Sv * 129X1/SvJ	Sp8^tm1Smb/Sp8^tm1Smb	Not Specified	olfactory pit, lateral nasal prominences, medial nasal prominences
-	Other lane(s) in blot assay different gene(s)

Notes:
(a) Significantly reduced expression compared with the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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