Probe:	Msx2 probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal cardiac muscle	Present	Regionally restricted	1B , 1A	Expression is in the wall and junction.
TS17: pericardium	Present		1B , 1A
TS17: endocardial cushion	Present	Regionally restricted	1B , 1A	Expression is in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: atrioventricular valve primordium	Present	Regionally restricted	2A , 2B	Expression is in the myocardium.
TS20: atrioventricular canal cardiac muscle	Present	Regionally restricted	2A , 2B
TS20: atrioventricular canal	Present	Regionally restricted	2A , 2B	Expression is in the myocardium of the atrioventricular junction.
TS20: endocardial cushion	Present	Regionally restricted	2B , 2A	Expression is in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: atrioventricular valve	Present	Regionally restricted	3A , 3B	Expression is at the base.
TS21: atrioventricular canal cardiac muscle	Present	Regionally restricted	3A , 3B	Expression is at the junction.
TS21: endocardial cushion	Weak	Regionally restricted	3A , 3B	Expression is in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: endocardial cushion	Strong	Graded	4B , 4A	Expression is in the condensed mesenchymal cells in the periphery of the endocardial cushion and decreased gradually from the more differentiated to the immature mesenchyme.
TS20: atrioventricular valve primordium	Present		4B , 4A

Structure	Level	Pattern	Image	Note
TS21: atrioventricular valve	Present	Regionally restricted	5B , 5A	Expression is in the myocardium of the base of the valve and some adjacent mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS24: endocardial cushion	Absent