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Gene Expression Data
Assay Details
Assay
Reference: J:226322 Roux M, et al., Hoxb1 regulates proliferation and differentiation of second heart field progenitors in pharyngeal mesoderm and genetically interacts with Hoxa1 during cardiac outflow tract development. Dev Biol. 2015 Oct 15;406(2):247-58
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7345840
Gene symbol: Hoxb1
Gene name: homeobox B1
Reporter: GFP
Antibody: anti-GFP (Invitrogen)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen S1B/C: embryonic day 9.5; Hoxb1tm5Mrc/Hoxb1+ (more )
Note: Double labeled: green - Hoxb1; red - Isl1 (assay MGI:7345836).
Structure Level Pattern Image Note
TS15: secondary heart field Present S1C , S1B Coexpressed with Isl1.
TS15: branchial arch endoderm Present S1B , S1C Coexpressed with Isl1 in pharyngeal endoderm.
Specimen S1E/F: embryonic day 9.5; Hoxb1tm5Mrc/Hoxb1+ (more )
Note: Double labeled: green - Hoxb1; red - Isl1 (assay MGI:7345836).
Structure Level Pattern Image Note
TS15: secondary heart field Present S1E , S1F Coexpressed with Isl1.
TS15: branchial arch endoderm Present S1F , S1E Coexpressed with Isl1 in pharyngeal endoderm.
Specimen S5B: embryonic day 9.5; Hoxb1tm5Mrc/Hoxb1+ (more )
Structure Level Pattern Image Note
TS15: branchial arch endoderm Present S5B
TS15: branchial arch mesenchyme Present Regionally restricted S5B Expressed in ventral pharyngeal mesoderm in the dorsal pericardial wall.
TS15: outflow tract Present Regionally restricted S5B Expressed in the inferior wall of the distal region.
Specimen S5C: embryonic day 9.5; Hoxb1tm5Mrc/Hoxb1tm5Mrc (more )
Structure Level Pattern Image Note
TS15: branchial arch endoderm Present S5C
TS15: branchial arch mesenchyme Present Regionally restricted S5C Expressed in ventral pharyngeal mesoderm in the dorsal pericardial wall.
TS15: outflow tract Present Regionally restricted S5C Expressed in the inferior wall of the distal region.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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