Gene Expression Data
Assay Details
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Assay
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Reference:
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J:182446
Kon N, et al., Inactivation of arf-bp1 induces p53 activation and diabetic phenotypes in mice. J Biol Chem. 2012 Feb 10;287(7):5102-11
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7343922
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Gene symbol:
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Ins1
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Gene name:
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insulin I
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Antibody:
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anti-insulin
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Assay notes:
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The specificity of the antibody used was not detailed; both family members have been annotated.
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Results
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Specimen
3Aii:
postnatal month 1; Huwe1tm1Wgu/Y
(more  )
Specimen
3Aii:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Huwe1tm1Wgu/Y
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Age:
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postnatal month 1
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
type B pancreatic cell |
Present |
Regionally restricted |
3Aii
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Expressed in the core of the islet.
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Specimen
3Av:
(close )
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Genetic Background:
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involves: C57BL/6J * CBA/J
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Mutant Allele(s):
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Huwe1tm1Wgu/Y
Tg(Ins2-cre)23Herr/0
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Age:
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postnatal month 1
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
type B pancreatic cell |
Present |
Regionally restricted |
3Av
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Similar as in the control, expressed in the core of the islet.
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Specimen
4Bi:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Huwe1tm1Wgu/Y
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Age:
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postnatal month 1
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
type B pancreatic cell |
Present |
Regionally restricted |
4Bi
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Expressed in round shape and center-located beta-cells.
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Specimen
4Bii:
postnatal month 6; Huwe1tm1Wgu/Y
(more )
Specimen
4Bii:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Huwe1tm1Wgu/Y
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Age:
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postnatal month 6
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
type B pancreatic cell |
Present |
Regionally restricted |
4Bii
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Expressed in round shape and center-located beta-cells.
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Specimen
4Biii:
postnatal month 9; Huwe1tm1Wgu/Y
(more )
Specimen
4Biii:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Huwe1tm1Wgu/Y
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Age:
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postnatal month 9
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
type B pancreatic cell |
Present |
Regionally restricted |
4Biii
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Expressed in round shape and center-located beta-cells.
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Specimen
4Bvii:
(close )
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Genetic Background:
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involves: C57BL/6J * CBA/J
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Mutant Allele(s):
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Huwe1tm1Wgu/Y
Tg(Ins2-cre)23Herr/0
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Age:
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postnatal month 1
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
type B pancreatic cell |
Present |
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4Bvii
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Similar as in the control, expressed in the core of the islet.
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Specimen
4Bviii:
(close )
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Genetic Background:
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involves: C57BL/6J * CBA/J
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Mutant Allele(s):
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Huwe1tm1Wgu/Y
Tg(Ins2-cre)23Herr/0
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Age:
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postnatal month 6
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
type B pancreatic cell |
Present |
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4Bviii
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The morphology became increasingly irregular with frequent infiltration of alpha-cells in the middle of the islets.
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Specimen
4Bix:
(close )
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Genetic Background:
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involves: C57BL/6J * CBA/J
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Mutant Allele(s):
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Huwe1tm1Wgu/Y
Tg(Ins2-cre)23Herr/0
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Age:
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postnatal month 9
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
type B pancreatic cell |
Weak |
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4Bix
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Near absence of staining, with worsened disruption of the islet structure.
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Specimen
6B:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Huwe1tm1Wgu/Y
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Age:
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postnatal month 9
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
type B pancreatic cell |
Present |
Regionally restricted |
6B
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Expressed in the core of the islet.
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Specimen
6D:
(close )
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Genetic Background:
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involves: C57BL/6J * CBA/J
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Mutant Allele(s):
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Huwe1tm1Wgu/Y
Tg(Ins2-cre)23Herr/0
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Age:
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postnatal month 9
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
type B pancreatic cell |
Weak |
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6D
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Near absence of staining, with disruption of the islet structure.
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Specimen
6F:
(close )
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Genetic Background:
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involves: C57BL/6J * CBA/J
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Mutant Allele(s):
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Huwe1tm1Wgu/Y
Trp53tm1Thl/Trp53tm1Thl
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Age:
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postnatal month 9
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
type B pancreatic cell |
Present |
Regionally restricted |
6F
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Expressed in the core of the islet.
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Specimen
6H:
(close )
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: islets of Langerhans |
type B pancreatic cell |
Present |
Regionally restricted |
6H
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Similar as in the wild-type control, expressed in the core of the islet.
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