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Gene Expression Data
Assay Details
Assay
Reference: J:216076 Hou C, et al., Abnormal cerebellar development and Purkinje cell defects in Lgl1-Pax2 conditional knockout mice. Dev Biol. 2014 Nov 1;395(1):167-81
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7341508
Gene symbol: Pcna
Gene name: proliferating cell nuclear antigen
Antibody: anti-PCNA
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 6G/H: embryonic day 14.5 (more )
Note: After 30 minutes pulse labeling of BrdU. Double labeled: red - Pcna; green - anti-BrdU antibody.
Structure Cell Type Level Pattern Image Note
TS22: cerebellum neural progenitor cell Present 6H , 6G
Specimen 6J/K: embryonic day 14.5; Llgl1tm1Vv/Llgl1tm1Vv, Tg(Pax2-cre)1Akg/0 (more )
Note: Conditional mutant. After 30 minutes pulse labeling of BrdU. Double labeled: red - Pcna; green - anti-BrdU antibody.
Structure Cell Type Level Pattern Image Note
TS22: cerebellum neural progenitor cell Present 6J , 6K There was no difference in the length of the cell cycle (coexpressed with BrdU) between wild-type and mutant cerebellar primordium.
Specimen 6M/N: embryonic day 14.5 (more )
Note: After 24 hours pulse labeling of BrdU. Double labeled: red - Pcna; green - anti-BrdU antibody.
Structure Cell Type Level Pattern Image Note
TS22: cerebellum neural progenitor cell Present 6M , 6N
TS22: cerebellum ventricular layer neural progenitor cell Present 6N , 6M Co-stained with anti-BrdU.
Specimen 6P/Q: embryonic day 14.5; Llgl1tm1Vv/Llgl1tm1Vv, Tg(Pax2-cre)1Akg/0 (more )
Note: Conditional mutant. After 24 hours pulse labeling of BrdU. Double labeled: red - Pcna; green - anti-BrdU antibody.
Structure Cell Type Level Pattern Image Note
TS22: cerebellum neural progenitor cell Present 6P , 6Q More cells remained in the cell cycle in mutant (coexpressed with BrdU) than in wild type embryos. Not only the areas around the ventricular zone, but also the inner layer of the cerebellar primodium was labelled by both.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/28/2026
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