Gene Expression Data
Assay Details
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Assay
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Reference:
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J:327413
Gillnas S, et al., Severe cerebellar malformations in mutant mice demonstrate a role for PDGF-C/PDGFRalpha signalling in cerebellar development. Biol Open. 2022 Aug 15;11(8):bio059431
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7341415
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Gene symbol:
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Pdgfc
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Gene name:
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platelet-derived growth factor, C polypeptide
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
E9.5 not shown:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pdgfctm1Nagy/Pdgfc+
Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pdgfctm1Nagy/Pdgfc+
Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pdgfctm1Nagy/Pdgfc+
Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cerebellum primordium |
Present |
Regionally restricted |
6B
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Expression first identified at E12.5 in caudal part of cerebellar primordium.
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Specimen
6C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pdgfctm1Nagy/Pdgfc+
Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pdgfctm1Nagy/Pdgfctm1Nagy
Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pdgfctm1Nagy/Pdgfctm1Nagy
Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pdgfctm1Nagy/Pdgfctm1Nagy
Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: rhombic lip |
Very strong |
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6F
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Expression stronger than in control mice.
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Specimen
S1A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pdgfctm1Nagy/Pdgfc+
Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
S1B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pdgfctm1Nagy/Pdgfctm1Nagy
Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebellum |
Present |
Widespread |
S1B
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More widespread expression pattern suggesting ectopic migration of Pdgfc-expressing cells.
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Specimen
S1C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pdgfctm1Nagy/Pdgfc+
Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
S1D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pdgfctm1Nagy/Pdgfctm1Nagy
Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cerebellum |
Present |
Widespread |
S1D
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More widespread expression pattern suggesting ectopic migration of Pdgfc-expressing cells.
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Specimen
S1E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pdgfctm1Nagy/Pdgfc+
Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
S1F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pdgfctm1Nagy/Pdgfctm1Nagy
Pdgfratm11(EGFP)Sor/Pdgfra+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cerebellum |
Present |
Widespread |
S1F
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More widespread expression pattern suggesting ectopic migration of Pdgfc-expressing cells.
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