Gene Expression Data
Assay Details
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Assay
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Reference:
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J:324880
Inoue YU, et al., Targeting Neurons with Functional Oxytocin Receptors: A Novel Set of Simple Knock-In Mouse Lines for Oxytocin Receptor Visualization and Manipulation. eNeuro. 2022 Jan-Feb;9(1):ENEURO.0423-21.2022
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7336795
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Gene symbol:
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Oxtr
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Gene name:
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oxytocin receptor
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Reporter:
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other (see notes)
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Antibody:
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anti-PA tag (clone NZ-1) (016-25861)
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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Podoplanin, included in the allele construct, was used as the reporter in this assay.
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Results
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Specimen
2f:
(close  )
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Genetic Background:
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involves: C3H * C57BL/6J
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Mutant Allele(s):
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Oxtrem3Yinn/Oxtrem3Yinn
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Age:
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postnatal week 2
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Bregma -1.82 mm
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Specimen
2i,j:
(close )
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Genetic Background:
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involves: C3H * C57BL/6J
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Mutant Allele(s):
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Oxtrem3Yinn/Oxtrem3Yinn
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Age:
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postnatal week 2
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Oxtr reporter; magenta - Sst (assay MGI:7336804).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: barrel cortex |
cortical interneuron |
Present |
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2j
,
2i
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Expression is seen on the surface of the cell body and the neurites.
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| TS28: cortical layer IV |
cortical interneuron |
Present |
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2j
,
2i
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Expression is seen on the surface of the cell body and the neurites.
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)
Analysis Tools