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Gene Expression Data
Assay Details
Assay
Reference: J:326479 Wheaton BJ, et al., Alternative LIM homeodomain splice variants are dynamically regulated at key developmental steps in vertebrates. Dev Dyn. 2022 Jul;251(7):1223-1243
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7336120
Gene symbol: Lhx9
Gene name: LIM homeobox protein 9
Antibody: anti-pan-Lhx9
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 7C: embryonic day 11.5 (more )
Note: Double labeled: green - Lhx9 (pan-Lhx9 antibody); red - Lhx9 (Lhx9ab-specific antibody; assay MGI:7336121).
Structure Level Pattern Image Note
TS19: neural tube mantle layer Present Regionally restricted 7C left Expressed in the dorsal part of the gray matter in recently born dI1 neurons/dorsal mantle zone.
TS19: forelimb bud Present 7C left
Specimen 8A E10.5: embryonic day 10.5 (more )
Note: Double labeled: green - Lhx9 (pan-Lhx9 antibody); red - Lhx9 (Lhx9ab-specific antibody; assay MGI:7336121).
Structure Level Pattern Image Note
TS17: forelimb bud Present 8A-3 , 8A-2
Specimen 8A E11.5: embryonic day 11.5 (more )
Note: Double labeled: green - Lhx9 (pan-Lhx9 antibody); red - Lhx9 (Lhx9ab-specific antibody; assay MGI:7336121).
Structure Level Pattern Image Note
TS19: forelimb bud Present 8A-6 , 8A-5
Specimen 8A E12.5: embryonic day 12.5 (more )
Note: Double labeled: green - Lhx9 (pan-Lhx9 antibody); red - Lhx9 (Lhx9ab-specific antibody; assay MGI:7336121).
Structure Level Pattern Image Note
TS20: forelimb Present 8A-9 , 8A-8
Specimen 8B E10.5: embryonic day 10.5 (more )
Note: Double labeled: green - Lhx9 (pan-Lhx9 antibody); red - Lhx9 (Lhx9ab-specific antibody; assay MGI:7336121).
Structure Level Pattern Image Note
TS17: urogenital ridge Present 8B-3 , 8B-2
Specimen 8B E11.5: embryonic day 11.5 (more )
Note: Double labeled: green - Lhx9 (pan-Lhx9 antibody); red - Lhx9 (Lhx9ab-specific antibody; assay MGI:7336121).
Structure Level Pattern Image Note
TS19: urogenital ridge Present 8B-6 , 8B-5
Specimen 8B E12.5 left: embryonic day 12.5 (more )
Note: Double labeled: green - Lhx9 (pan-Lhx9 antibody); red - Lhx9 (Lhx9ab-specific antibody; assay MGI:7336121).
Structure Level Pattern Image Note
TS20: urogenital ridge Present 8B-11 , 8B-9
Specimen 8B E12.5 right: embryonic day 12.5 (more )
Note: Double labeled: green - Lhx9 (pan-Lhx9 antibody); red - Lhx9 (Lhx9ab-specific antibody; assay MGI:7336121).
Structure Level Pattern Image Note
TS20: urogenital ridge Ambiguous 8B-12 , 8B-10
Specimen 10A: embryonic day 10.5 (more )
Note: Brachial transverse sections. Double labeled: green - Lhx9 (pan-Lhx9 antibody); red - Lhx9 (Lhx9ab-specific antibody; assay MGI:7336121).
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted 10A right , 10A middle Expressed in the dorsal part of the gray matter in recently born dI1 neurons/dorsal mantle zone.
Specimen 10B: embryonic day 11.5 (more )
Note: Brachial transverse sections. Double labeled: green - Lhx9 (pan-Lhx9 antibody); red - Lhx9 (Lhx9ab-specific antibody; assay MGI:7336121).
Structure Level Pattern Image Note
TS19: neural tube mantle layer Present Regionally restricted 10B middle , 10B right Expressed in the dorsal part of the gray matter in recently born dI1 neurons/dorsal mantle zone.
TS19: neural tube lateral wall Present Regionally restricted 10B right , 10B middle Expressed in the deep dorsal horn/migrating stream of dI1 neurons. Expressed more broadly than Lhx9ab in about half the embryos examined.
Specimen 10C: embryonic day 12.5 (more )
Note: Brachial transverse sections Double labeled: green - Lhx9 (pan-Lhx9 antibody); red - Lhx9 (Lhx9ab-specific antibody; assay MGI:7336121).
Structure Level Pattern Image Note
TS20: spinal cord dorsal horn Present 10C middle , 10C right Strongest expression.
TS20: spinal cord mantle layer Weak Regionally restricted 10C right , 10C middle Expressed in the dorsal part of the gray matter in recently born dI1 neurons/dorsal mantle zone.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory