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Gene Expression Data
Assay Details
Assay
Reference: J:327232 Wu JPH, et al., The Transcription Factor Pou3f1 Sheds Light on the Development and Molecular Diversity of Glutamatergic Cerebellar Nuclear Neurons in the Mouse. Front Mol Neurosci. 2022;15:921901
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7336066
Gene symbol: Tbr1
Gene name: T-box brain transcription factor 1
Antibody: anti-Tbr1 (AB31940)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 6A: embryonic day 13.5 (more )
Note: Double labeled: green - Tbr1; red - Pou3f1 (assay MGI:7336061).
Structure Cell Type Level Pattern Image Note
TS21: cerebellum cerebellar neuron Present Regionally restricted 6A Expression in cerebellar nuclear (CN) neurons in same plate along medial-lateral axis and Pou3f1-expressing CN neurons, but Pou3f1-expressing CN neurons tend to cluster more ventrally.
Specimen 6B: embryonic day 15.5 (more )
Note: Double labeled: green - Tbr1; red - Pou3f1 (assay MGI:7336061).
Structure Cell Type Level Pattern Image Note
TS23: cerebellum cerebellar neuron Present Regionally restricted 6B Expression in cerebellar nuclear (CN) neurons in same plate along medial-lateral axis and Pou3f1-expressing CN neurons, but Pou3f1-expressing CN neurons tend to cluster more ventrally.
Specimen 6C: postnatal day 6 (more )
Note: Double labeled: green - Tbr1; red - Pou3f1 (assay MGI:7336061).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum cerebellar neuron Present Regionally restricted 6C Expression in cluster of in cerebellar nuclear (CN) neurons more medial than cluster of Pou3f1-expressing CN neurons.
Specimen 6D,D': postnatal day 6 (more )
Note: Double labeled: green - Tbr1; red - Pou3f1 (assay MGI:7336061).
Structure Level Pattern Image Note
TS28: cerebellum interpositus nucleus Absent 6D' , 6D
Specimen 6E,E': postnatal day 6 (more )
Note: Double labeled: green - Tbr1; red - Pou3f1 (assay MGI:7336061).
Structure Level Pattern Image Note
TS28: fastigial nucleus Present 6E , 6E' Expression primarily in fastigial nucleus.
Specimen 6F,F': postnatal day 6 (more )
Note: Double labeled: green - Tbr1; red - Pou3f1 (assay MGI:7336061).
Structure Level Pattern Image Note
TS28: dentate nucleus Absent 6F , 6F'
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory