Gene Expression Data
Assay Details
Assay
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Reference:
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J:327232
Wu JPH, et al., The Transcription Factor Pou3f1 Sheds Light on the Development and Molecular Diversity of Glutamatergic Cerebellar Nuclear Neurons in the Mouse. Front Mol Neurosci. 2022;15:921901
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7336061
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Gene symbol:
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Pou3f1
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Gene name:
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POU domain, class 3, transcription factor 1
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Results
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Specimen
2B:
embryonic day 10.5
(more )
Specimen
2B:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2C:
embryonic day 12.5
(more )
Specimen
2C:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebellum primordium |
Present |
Regionally restricted |
2C
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Between E12.5 and E15.5 expression in cells in nuclear transitory zone and nowhere else in the developing cerebellum.
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Specimen
2F:
postnatal day 6
(more )
Specimen
2F:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum white matter |
Present |
Regionally restricted |
2F
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Expression in clusters of cells in cerebellar white matter in the forming nuclear region.
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Specimen
2G:
postnatal day 6
(more )
Note: Double labeled: red - Pou3f1; green - vGluT2 (Slc17a6 assay MGI:7336062).
Specimen
2G:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pou3f1; green - vGluT2 (Slc17a6 assay MGI:7336062).
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Specimen
3A:
postnatal day 6
(more )
Note: Double labeled: red - Pou3f1; green - GAD67 (Gad1 assay MGI:7336063).
Specimen
3A:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
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Note:
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Double labeled: red - Pou3f1; green - GAD67 (Gad1 assay MGI:7336063).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: brain |
Present |
Regionally restricted |
3A
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No co-expression with GAD67 (Gad1).
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TS28: cerebellum white matter |
Present |
Regionally restricted |
3D
,
3B
,
3A
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Expression in cells not surrounded by GAD67 (Gad1)-expressing puncta.
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Specimen
4A:
embryonic day 12.5
(more )
Note: Double labeled: red - Pou3f1; green - Atoh1 (assay MGI:7336064).
Specimen
4A:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
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Note:
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Double labeled: red - Pou3f1; green - Atoh1 (assay MGI:7336064).
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Specimen
4B:
embryonic day 12.5
(more )
Note: Double labeled: red - Pou3f1; green - Atoh1 (assay MGI:7336064).
Specimen
4B:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou3f1; green - Atoh1 (assay MGI:7336064).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: rhombic lip |
Absent |
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4B
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No expression in posterior and lateral part of rhombic lip.
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Specimen
4C:
embryonic day 12.5
(more )
Note: Double labeled: red - Pou3f1; green - Atoh1 (assay MGI:7336064).
Specimen
4C:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 12.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou3f1; green - Atoh1 (assay MGI:7336064).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebellum primordium |
Present |
Regionally restricted |
4C
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Expression co-localized with Atoh1 in part of subpial stream proximal to rhombic lip.
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Specimen
4D:
embryonic day 12.5
(more )
Note: Double labeled: red - Pou3f1; green - Atoh1 (assay MGI:7336064).
Specimen
4D:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou3f1; green - Atoh1 (assay MGI:7336064).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebellum primordium |
Present |
Regionally restricted |
4D
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Expression in more distal parts of subpial stream.
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Specimen
5A:
embryonic day 13.5
(more )
Note: Double labeled: red - Pou3f1; green - Pax6 (assay MGI:7336065).
Specimen
5A:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou3f1; green - Pax6 (assay MGI:7336065).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebellum |
Present |
Regionally restricted |
5A
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Expression predomininantly in nuclear transitory zone; not co-localized with Pax6 expression in rhombic lip and beginning of subpial stream.
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TS21: rhombic lip |
Absent |
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5A
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Specimen
5A":
embryonic day 13.5
(more )
Note: Double labeled: red - Pou3f1; green - Pax6 (assay MGI:7336065).
Specimen
5A":
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou3f1; green - Pax6 (assay MGI:7336065).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebellum |
Present |
Regionally restricted |
5A'
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Expression in cells localized to the nuclear transitory zone.
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Specimen
5A'':
embryonic day 13.5
(more )
Note: Double labeled: red - Pou3f1; green - Pax6 (assay MGI:7336065).
Specimen
5A'':
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou3f1; green - Pax6 (assay MGI:7336065).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: rhombic lip |
Absent |
|
5A''
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Expression not co-localized with Pax6 in rhombic lip and beginning of subpial stream.
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Specimen
6A:
embryonic day 13.5
(more )
Note: Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
Specimen
6A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebellum |
Present |
Regionally restricted |
6A
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Expression in cells located more medially than Tbr1-expressing cells.
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Specimen
6B:
embryonic day 15.5
(more )
Note: Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
Specimen
6B:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
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Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: cerebellum |
cerebellar neuron |
Present |
Regionally restricted |
6B
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Expression in cerebellar nuclear (CN) neurons in same plate along medial-lateral axis and Tbr1-expressing CN neurons, but Pou3f1-expressing CN neurons tend to cluster more ventrally.
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Specimen
6C:
postnatal day 6
(more )
Note: Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
Specimen
6C:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Note:
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Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
cerebellar neuron |
Present |
Regionally restricted |
6C
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Expression in cluster of in cerebellar nuclear (CN) neurons more lateral than cluster of Tbr1-expressing CN neurons.
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Specimen
6D,D':
postnatal day 6
(more )
Note: Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
Specimen
6D,D':
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
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Note:
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Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
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Specimen
6E,E':
postnatal day 6
(more )
Note: Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
Specimen
6E,E':
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
postnatal day 6
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Note:
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Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
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Specimen
6F,F':
postnatal day 6
(more )
Note: Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
Specimen
6F,F':
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
postnatal day 6
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
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Specimen
7B:
postnatal day 6
(more )
Note: Double labeled: red - Pou3f1; green - Brn2 (Pou3f2 assay MGI:7336068).
Specimen
7B:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
postnatal day 6
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Sex:
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Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou3f1; green - Brn2 (Pou3f2 assay MGI:7336068).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
7B
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Co-expression with Brn2 (Pou3f2). Some Pou3f1-expressing cells that are not labele by Brn2.
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Specimen
7C,C':
postnatal day 6
(more )
Note: Double labeled: red - Pou3f1; green - Brn2 (Pou3f2 assay MGI:7336068).
Specimen
7C,C':
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 6
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou3f1; green - Brn2 (Pou3f2 assay MGI:7336068).
|
Specimen
7D,D':
postnatal day 6
(more )
Note: Double labeled: red - Pou3f1; green - Brn2 (Pou3f2 assay MGI:7336068).
Specimen
7D,D':
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 6
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Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou3f1; green - Brn2 (Pou3f2 assay MGI:7336068).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: dentate nucleus |
Present |
|
7D
,
7D'
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Co-expression with (Pou3f2) in dentate nucleus. Cohort of Brn2+/Pou3f1- cells.
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Specimen
9A:
postnatal day 6
(more )
Note: Double labeled: red - Pou3f1; green - Irx3 (assay MGI:7336070).
Specimen
9A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou3f1; green - Irx3 (assay MGI:7336070).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
9A
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Co-expression with Irx3.
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Specimen
9B,B':
postnatal day 6
(more )
Note: Double labeled: red - Pou3f1; green - Irx3 (assay MGI:7336070).
Specimen
9B,B':
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou3f1; green - Irx3 (assay MGI:7336070).
|
Specimen
9C,C':
postnatal day 6
(more )
Note: Double labeled: red - Pou3f1; green - Irx3 (assay MGI:7336070).
Specimen
9C,C':
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou3f1; green - Irx3 (assay MGI:7336070).
|
|