Probe:	Vax2 probe9
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: optic stalk	Present	Regionally restricted	5B	Expression solely in ventral halves of optic stalk and optic vesicles without proximo-distal restruction.
TS15: optic vesicle	Present	Regionally restricted	5B	Expression solely in ventral halves of optic stalk and optic vesicles without proximo-distal restruction.

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present	Regionally restricted	5C	Expression in ventral part of optic vesicle; expression absent in dorsal part.

Structure	Level	Pattern	Image	Note
TS17: optic cup inner layer	Present		5E	Expression restricted to inner layer of optic cup.

Structure	Level	Pattern	Image	Note
TS19: neural retina	Present	Regionally restricted	5G	Expression restricted to inner layer of optic cup in ventral half.
TS19: pigmented retinal epithelium	Absent		5G	Expression undetectable in outer layer of optic cup.
TS19: optic stalk	Absent		5G	Expression in optic stalk diminished after E11.5.

Structure	Level	Pattern	Image	Note
TS22: neural retina	Present	Regionally restricted	5I	Expression persisted in ventral half of retina nuclear layers. Stronger expression in inner than in outer nuclear layer.
TS22: optic stalk	Absent		5I

Structure	Level	Pattern	Image	Note
TS22: neural retina	Present	Regionally restricted	5J	Expression persisted in ventral half of retina nuclear layers. Stronger expression in inner than in outer nuclear layer.
TS22: optic stalk	Absent		5J	No expression in nerve fibers.

Structure	Level	Pattern	Image	Note
TS24: retina inner nuclear layer	Present	Regionally restricted		Expression persisted in ventral half of retina nuclear layers; stronger expression in inner than in outer nuclear layer.
TS24: retina outer nuclear layer	Present	Regionally restricted		Expression persisted in ventral half of retina nuclear layers; stronger expression in inner than in outer nuclear layer.

Structure	Level	Pattern	Image	Note
TS27: retina	Present	Regionally restricted	5K	No expression in dorsal retina.

Structure	Level	Pattern	Image	Note
TS27: retina inner nuclear layer	Present	Regionally restricted	5L	Expression persisted in ventral half of retina nuclear layers; stronger expression in inner than in outer nuclear layer.
TS27: retina outer nuclear layer	Present	Regionally restricted	5L	Expression persisted in ventral half of retina nuclear layers; stronger expression in inner than in outer nuclear layer.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Present		5M
TS28: retina ganglion cell layer	Present		5M
TS28: retina inner nuclear layer	Present		5M
TS28: retina outer plexiform layer	Strong		5M	Outer plexiform layer intensely positive for expression compared to other layers.