Gene Expression Data
Assay Details
Assay
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Reference:
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J:63408
Yip JW, et al., Reelin controls position of autonomic neurons in the spinal cord. Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8612-6
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7335177
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Gene symbol:
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Reln
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Gene name:
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reelin
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Results
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Specimen
4a:
embryonic day 11.5
(more )
Specimen
4a:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
4a
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Expression was widely distributed in the ventral spinal cord. The only regions that are devoid of staining were the neuroepithelium and the ventrolateral spinal cord, where preganglionic and somatic motor neurons are located.
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Specimen
4b:
embryonic day 12.0
(more )
Note: Double labeled: red - Reln; green - dextran amine.
Specimen
4b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Double labeled: red - Reln; green - dextran amine.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
|
Present |
Regionally restricted |
4b
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Expression was present adjacent to migrating preganglionic neurons (labeled by retrograde dextran amine) in the ventral spinal cord.
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TS20: spinal cord |
sympathetic neuron |
Absent |
|
4b
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Preganglionic sympathetic neurons of the ventrolateral spinal cord were not immunostained.
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Specimen
4c:
embryonic day 12.5
(more )
Specimen
4c:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
4c
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Expression was located more dorsally in the spinal cord.
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Specimen
4d:
embryonic day 14.0
(more )
Note: Double labeled: red - Reln; green - nitric oxide synthase.
Specimen
4d:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Reln; green - nitric oxide synthase.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: spinal cord |
|
Present |
Regionally restricted |
4d
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Expression was located more dorsally in the spinal cord, but it was adjacent to the preganglionic neurons (labeled with nitric oxide synthase immunostaining).
|
TS22: spinal cord |
sympathetic neuron |
Absent |
|
4d
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Preganglionic sympathetic neurons were not immunostained.
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