Antibody:	anti-Calbindin D28k (CB-38)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present	Widespread	8A , 8B , 8C	A continuous cell layer was seen.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present	Regionally restricted	8F , 8E , 8D	Parasagittal zones devoid of immunoreactivity throughout the cerebellum indicative of Purkinje cell loss were observed. In the vermis and paravermal zones the immunonegative zones were sagittaly distributed in narrow gaps; a greater extension at the hemispheres with some surviving Purkinje cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present	Widespread	8G	Purkinje cells somata aligned in a continuous row, with dendritic trees fulfill the molecular layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present	Spotted	8H	Presence of wide spaces lacking Purkinje cell somata and dendritic debris through the molecular layer, distribute differently according a medio-lateral gradient.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		10A	Fine grained morphology of normal Purkinje cells axonal plexuses was seen.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		10B	Rounded thickenings resembling axonal torpedoes were observed.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		10C	Rounded thickenings resembling axonal torpedoes were observed.