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Gene Expression Data
Assay Details
Assay
Reference: J:270680 Han X, et al., The transcription factor NKX2-3 mediates p21 expression and ectodysplasin-A signaling in the enamel knot for cusp formation in tooth development. J Biol Chem. 2018 Sep 21;293(38):14572-14584
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7334988
Gene symbol: Nkx2-3
Gene name: NK2 homeobox 3
Antibody: Nkx2-3 antibody
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1E (top) upper 1st molar E13: embryonic day 13.0 (more )
Note: Double labeled: green - Nkx2-3; red - perlecan (Hspg2 assay MGI:7334990).
Structure Level Pattern Image Note
TS21: tooth bud Present 1E (top) upper 1st molar E13 Expression from bud stage.
TS21: upper jaw molar Present 1E (top) upper 1st molar E13 Expression from bud stage.
Specimen 1E (top) upper 1st molar E14: embryonic day 14.0 (more )
Note: Double labeled: green - Nkx2-3; red - perlecan (Hspg2 assay MGI:7334990).
Structure Level Pattern Image Note
TS22: cap stage tooth Present 1E (top) upper 1st molar E14 Expression continues at cap stage.
TS22: upper jaw molar Present 1E (top) upper 1st molar E14 Expression continues at cap stage.
Specimen 1E (top) upper 1st molar E16: embryonic day 16.0 (more )
Note: Double labeled: green - Nkx2-3; red - perlecan (Hspg2 assay MGI:7334990).
Structure Level Pattern Image Note
TS24: bell stage tooth Present Regionally restricted 1E (top) upper 1st molar E16 At bell stage, expression in second enamel knot.
TS24: upper jaw molar Present Regionally restricted 1E (top) upper 1st molar E16 At bell stage, expression in second enamel knot.
TS24: secondary enamel knot Present Regionally restricted 1E (top) upper 1st molar E16
Specimen 1E (top) upper 1st molar E14 enlargement: embryonic day 14.0 (more )
Note: Double labeled: green - Nkx2-3; red - perlecan (Hspg2 assay MGI:7334990).
Structure Level Pattern Image Note
TS22: primary enamel knot Present 1E (top) upper 1st molar E14 enlargement Expression mainly at primary knot area.
Specimen 1E (top) lower 1st molar E13: embryonic day 13.0 (more )
Note: Double labeled: green - Nkx2-3; red - perlecan (Hspg2 assay MGI:7334990).
Structure Level Pattern Image Note
TS21: lower jaw molar Present 1E (top) lower 1st molar E13 Expression from bud stage.
TS21: tooth bud Present 1E (top) lower 1st molar E13 Expression from bud stage.
Specimen 1E (top) lower 1st molar E14: embryonic day 14.0 (more )
Note: Double labeled: green - Nkx2-3; red - perlecan (Hspg2 assay MGI:7334990).
Structure Level Pattern Image Note
TS22: cap stage tooth Present 1E (top) lower 1st molar E14 Expression continues at cap stage.
TS22: lower jaw molar Present 1E (top) lower 1st molar E14 Expression continues at cap stage.
Specimen 1E (top) lower 1st molar E16: embryonic day 16.0 (more )
Note: Double labeled: green - Nkx2-3; red - perlecan (Hspg2 assay MGI:7334990).
Structure Level Pattern Image Note
TS24: bell stage tooth Present Regionally restricted 1E (top) lower 1st molar E16 At bell stage, expression in second enamel knot.
TS24: lower jaw molar Present Regionally restricted 1E (top) lower 1st molar E16 At bell stage, expression in second enamel knot.
TS24: secondary enamel knot Present 1E (top) lower 1st molar E16
Specimen 1E (top) lower 1st molar E14 enlargement: embryonic day 14.0 (more )
Note: Double labeled: green - Nkx2-3; red - perlecan (Hspg2 assay MGI:7334990).
Structure Level Pattern Image Note
TS22: primary enamel knot Present 1E (top) lower 1st molar E14 enlargement Expression mainly in primary knot area.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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