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Gene Expression Data
Assay Details
Assay
Reference: J:316299 Freire AG, et al., Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate. Stem Cell Reports. 2017 Jul 11;9(1):136-148
Assay type: RNA in situ
MGI Accession ID: MGI:7332809
Gene symbol: Hes5
Gene name: hes family bHLH transcription factor 5
Probe: Hes5 probe24
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1C ES: embryonic day 6.5 (more )
Note: early streak stage
Structure Level Pattern Image Note
TS9: embryo mesoderm Present 1C ES
Specimen 1Ca: embryonic day 6.5 (more )
Note: early streak stage
Structure Level Pattern Image Note
TS9: embryo mesoderm Present 1Ca
Specimen 1Cb: embryonic day 6.5 (more )
Note: early streak stage
Structure Level Pattern Image Note
TS9: embryo mesoderm Present 1Cb
Specimen 1C LB: embryonic day 7.5 (more )
Note: late bud stage
Structure Level Pattern Image Note
TS11: embryo Weak 1C LB Expression was dramatically downregulated (3 out of 8 samples), or not expressed (5 out of 8 samples) in late bud embryos.
Specimen not shown LB: embryonic day 7.5 (more )
Note: late bud stage
Structure Level Pattern Image Note
TS11: embryo Absent Expression was dramatically downregulated (3 out of 8 samples), or not expressed (5 out of 8 samples) in late bud embryos.
Specimen S1B ES: embryonic day 6.5 (more )
Note: early streak stage
Structure Level Pattern Image Note
TS9: embryo mesoderm Present S1B ES
Specimen S1B MS: embryonic day 7.0 (more )
Note: mid streak stage
Structure Level Pattern Image Note
TS10: mesoderm Present S1B MS
Specimen S1B LS: embryonic day 7.0 (more )
Note: late streak stage
Structure Level Pattern Image Note
TS10: embryo ectoderm Present S1B LS
Specimen S1B EB: embryonic day 7.0 (more )
Note: early bud stage
Structure Level Pattern Image Note
TS10: amniotic fold Present Regionally restricted S1B EB Expression was detected in the posterior amniotic fold.
TS10: embryo Weak Regionally restricted S1B EB Expression was detected in the head fold.
Specimen S1B LB: embryonic day 7.5 (more )
Note: late bud stage
Structure Level Pattern Image Note
TS11: embryo Weak S1B LB Expression was dramatically downregulated (3 out of 8 samples), or not expressed (5 out of 8 samples) in late bud embryos.
TS11: neural ectoderm Weak S1B LB
Specimen S1B HF top: embryonic day 7.5 (more )
Note: headfold stage
Structure Level Pattern Image Note
TS12: embryo Present Regionally restricted S1B HF top Expression was detected in the head fold.
Specimen S1B HF bottom: embryonic day 7.5 (more )
Note: headfold stage
Structure Level Pattern Image Note
TS12: unsegmented mesenchyme Present S1B HF bottom
Specimen S1B HF right: embryonic day 7.5 (more )
Note: headfold stage
Structure Level Pattern Image Note
TS12: embryo Present Regionally restricted S1B HF right Expression was detected in the head fold.
TS12: unsegmented mesenchyme Present S1B HF right
TS12: primitive heart tube Absent S1B HF right
Specimen S1B E8.5: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: embryo Present Regionally restricted S1B E8.5 Expression was detected in the head fold.
TS13: future hindbrain Present S1B E8.5
TS13: future midbrain Present S1B E8.5
TS13: somite Present S1B E8.5
TS13: unsegmented mesenchyme Present S1B E8.5
Specimen S1B E9.5: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: embryo Present Regionally restricted S1B E9.5 Expression was detected in the head fold.
TS15: future hindbrain Present S1B E9.5
TS15: future midbrain Present S1B E9.5
TS15: somite Present S1B E9.5
TS15: unsegmented mesenchyme Present S1B E9.5
Specimen not shown E8.5: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: neural tube Present
Specimen not shown E9.5: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: neural tube Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
01/20/2026
MGI 6.24 		The Jackson Laboratory