Gene Expression Data
Assay Details
Assay
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Reference:
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J:216287
Czajkowski MT, et al., Divergent and conserved roles of Dll1 signaling in development of craniofacial and trunk muscle. Dev Biol. 2014 Nov 15;395(2):307-16
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7331551
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Gene symbol:
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Pax7
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Gene name:
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paired box 7
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Antibody:
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Anti-Pax7
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Detection system:
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Secondary antibody
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Results
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Specimen
6A:
embryonic day 18.0
(more )
Note: Double labeled: red - Pax7; green - laminin.
Specimen
6A:
(close )
Genetic Background:
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Not Specified
|
Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax7; green - laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: masseter |
skeletal muscle myoblast |
Present |
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6A
|
87% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
TS26: masseter |
skeletal muscle satellite cell |
Present |
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6A
|
87% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
Specimen
6A':
embryonic day 18.0
(more )
Note: Double labeled: red - Pax7; green - laminin.
Specimen
6A':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pax7; green - laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: tongue extrinsic muscle |
skeletal muscle myoblast |
Present |
|
6A'
|
85% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
TS26: tongue extrinsic muscle |
skeletal muscle satellite cell |
Present |
|
6A'
|
85% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
Specimen
6A":
embryonic day 18.0
(more )
Note: Double labeled: red - Pax7; green - laminin.
Specimen
6A":
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pax7; green - laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: extrinsic ocular muscle |
skeletal muscle myoblast |
Present |
|
6A"
|
80% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
TS26: extrinsic ocular muscle |
skeletal muscle satellite cell |
Present |
|
6A"
|
80% of the Pax7+ muscle progenitor cells were located below the basal lamina.
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Specimen
6B:
embryonic day 18.0; Dll1tm1Gos/ Dll1tm2Gos
(more )
Note: Double labeled: red - Pax7; green - laminin.
Specimen
6B:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Dll1tm1Gos/Dll1tm2Gos
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pax7; green - laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: masseter |
skeletal muscle myoblast |
Ambiguous |
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6B
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Positive cells were very rare.
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Specimen
6B':
embryonic day 18.0; Dll1tm1Gos/ Dll1tm2Gos
(more )
Note: Double labeled: red - Pax7; green - laminin.
Specimen
6B':
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Dll1tm1Gos/Dll1tm2Gos
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Age:
|
embryonic day 18.0
|
Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pax7; green - laminin.
|
Specimen
6B":
embryonic day 18.0; Dll1tm1Gos/ Dll1tm2Gos
(more )
Note: Double labeled: red - Pax7; green - laminin.
Specimen
6B":
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Dll1tm1Gos/Dll1tm2Gos
|
Age:
|
embryonic day 18.0
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Double labeled: red - Pax7; green - laminin.
|
Specimen
6C:
embryonic day 18.0; Myod1tm1Jae/ Myod1tm1Jae
(more )
Note: Double labeled: red - Pax7; green - laminin.
Specimen
6C:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ
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Mutant Allele(s):
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Myod1tm1Jae/Myod1tm1Jae
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Age:
|
embryonic day 18.0
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pax7; green - laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: masseter |
skeletal muscle myoblast |
Present |
|
6C
|
Overall numbers of positive cells were increased, and many of the supernumerous cells located to the interstitial space. 63% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
TS26: masseter |
skeletal muscle satellite cell |
Present |
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6C
|
Overall numbers of positive cells were increased, and many of the supernumerous cells located to the interstitial space. 63% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
Specimen
6C':
embryonic day 18.0; Myod1tm1Jae/ Myod1tm1Jae
(more )
Note: Double labeled: red - Pax7; green - laminin.
Specimen
6C':
(close )
Genetic Background:
|
involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ
|
Mutant Allele(s):
|
Myod1tm1Jae/Myod1tm1Jae
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Age:
|
embryonic day 18.0
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pax7; green - laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: tongue extrinsic muscle |
skeletal muscle myoblast |
Present |
|
6C'
|
Overall numbers of positive cells were increased, and many of the supernumerous cells located to the interstitial space. 65% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
TS26: tongue extrinsic muscle |
skeletal muscle satellite cell |
Present |
|
6C'
|
Overall numbers of positive cells were increased, and many of the supernumerous cells located to the interstitial space. 65% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
Specimen
6C":
embryonic day 18.0; Myod1tm1Jae/ Myod1tm1Jae
(more )
Note: Double labeled: red - Pax7; green - laminin.
Specimen
6C":
(close )
Genetic Background:
|
involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ
|
Mutant Allele(s):
|
Myod1tm1Jae/Myod1tm1Jae
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pax7; green - laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: extrinsic ocular muscle |
skeletal muscle myoblast |
Present |
|
6C"
|
Overall numbers of positive cells were increased, and many of the supernumerous cells located to the interstitial space. 69% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
TS26: extrinsic ocular muscle |
skeletal muscle satellite cell |
Present |
|
6C"
|
Overall numbers of positive cells were increased, and many of the supernumerous cells located to the interstitial space. 69% of the Pax7+ muscle progenitor cells were located below the basal lamina.
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Specimen
6D:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ
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Mutant Allele(s):
|
Dll1tm1Gos/Dll1tm2Gos Myod1tm1Jae/Myod1tm1Jae
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Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pax7; green - laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: masseter |
skeletal muscle myoblast |
Present |
|
6D
|
63% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
TS26: masseter |
skeletal muscle satellite cell |
Present |
|
6D
|
63% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
Specimen
6D':
(close )
Genetic Background:
|
involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ
|
Mutant Allele(s):
|
Dll1tm1Gos/Dll1tm2Gos Myod1tm1Jae/Myod1tm1Jae
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pax7; green - laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: tongue extrinsic muscle |
skeletal muscle myoblast |
Present |
|
6D'
|
51% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
TS26: tongue extrinsic muscle |
skeletal muscle satellite cell |
Present |
|
6D'
|
51% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
Specimen
6D":
(close )
Genetic Background:
|
involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ
|
Mutant Allele(s):
|
Dll1tm1Gos/Dll1tm2Gos Myod1tm1Jae/Myod1tm1Jae
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pax7; green - laminin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: extrinsic ocular muscle |
skeletal muscle myoblast |
Present |
|
6D"
|
45% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
TS26: extrinsic ocular muscle |
skeletal muscle satellite cell |
Present |
|
6D"
|
45% of the Pax7+ muscle progenitor cells were located below the basal lamina.
|
|