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Gene Expression Data
Assay Details
Assay
Reference: J:325976 Piprek RP, et al., Desmoplakin (Dsp) conditional knockout in NR5A1+ somatic cells affects germ cell survival in developing mouse gonads. Reproduction. 2022 Feb 24;163(4):199-207
Assay type: RT-PCR
MGI Accession ID: MGI:7330120
Gene symbol: Dsp
Gene name: desmoplakin
Probe: Dsp-pE, Dsp-pF
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Actb. Genetic background of control specimens was either Dsp1fl/fl or Nr5a1-Cre.
Results Image: 1G
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
E10.5 C E10.5 TS17: gonad primordium Present Not Specified; total RNA Not Specified Male
E10.5 KO E10.5 TS17: gonad primordium Present Not Specified; total RNA involves: 129 * FVB Dsptm1Efu/Dsptm1Efu, Tg(Nr5a1-cre)7Lowl/0Male Conditional mutant.
E11.5 C E11.5 TS19: gonad Present Not Specified; total RNA Not Specified Male
E11.5 KO E11.5 TS19: gonad Present (a) Not Specified; total RNA involves: 129 * FVB Dsptm1Efu/Dsptm1Efu, Tg(Nr5a1-cre)7Lowl/0Male Conditional mutant.
E13.5 C E13.5 TS21: testis Strong Not Specified; total RNA Not Specified Male
E13.5 KO E13.5 TS21: testis Weak (b) Not Specified; total RNA involves: 129 * FVB Dsptm1Efu/Dsptm1Efu, Tg(Nr5a1-cre)7Lowl/0Male Conditional mutant.
E16.5 C E16.5 TS24: testis Strong Not Specified; total RNA Not Specified Male
E16.5 KO E16.5 TS24: testis Weak (b) Not Specified; total RNA involves: 129 * FVB Dsptm1Efu/Dsptm1Efu, Tg(Nr5a1-cre)7Lowl/0Male Conditional mutant.
2 dpp C P2 TS27: testis Strong Not Specified; total RNA Not Specified Male
2 dpp KO P2 TS27: testis Weak (b) Not Specified; total RNA involves: 129 * FVB Dsptm1Efu/Dsptm1Efu, Tg(Nr5a1-cre)7Lowl/0Male Conditional mutant.
Notes:
(a) Expression significantly lower than control from E11.5 onward.
(b) Declining expression in mutant gonads. Low expression still detectable
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory