Gene Expression Data
Assay Details
Assay
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Reference:
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J:308556
Goss G, et al., Distinct Fibroblast Lineages Give Rise to NG2+ Pericyte Populations in Mouse Skin Development and Repair. Front Cell Dev Biol. 2021;9:675080
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7327135
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Gene symbol:
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Nes
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Gene name:
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nestin
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Results
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Specimen
1C E14.5:
embryonic day 14.5
(more )
Note: Double labeled: red - Nes; green - Pecam1 (assay MGI:7327129).
Specimen
1C E14.5:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Nes; green - Pecam1 (assay MGI:7327129).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: blood vessel |
pericyte |
Present |
Regionally restricted |
1C E14.5
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The proportion of Nestin positive blood vessel associated cells in back skin was approximately 20-30%.
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Specimen
1C E16.5:
embryonic day 16.5
(more )
Note: Double labeled: red - Nes; green - Pecam1 (assay MGI:7327129).
Specimen
1C E16.5:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: red - Nes; green - Pecam1 (assay MGI:7327129).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: blood vessel |
pericyte |
Present |
Regionally restricted |
1C E16.5
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The proportion of Nestin positive blood vessel associated cells in back skin was approximately 20-30%.
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TS24: dermis |
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Present |
Regionally restricted |
1C E16.5
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Immunopositive dermal cells were present in the vicinity of blood vessels at E16.5.
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Specimen
1C E18.5:
embryonic day 18.5
(more )
Note: Double labeled: red - Nes; green - Pecam1 (assay MGI:7327129).
Specimen
1C E18.5:
(close )
Genetic Background:
|
C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: red - Nes; green - Pecam1 (assay MGI:7327129).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: blood vessel |
pericyte |
Present |
Regionally restricted |
1C E18.5
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The proportion of Nestin positive blood vessel associated cells in back skin was approximately 20-30%.
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Specimen
1C P50:
postnatal day 50
(more )
Note: Double labeled: red - Nes; green - Pecam1 (assay MGI:7327129).
Specimen
1C P50:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 50
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Nes; green - Pecam1 (assay MGI:7327129).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: blood vessel |
pericyte |
Present |
Regionally restricted |
1C P50
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The proportion of Nestin positive blood vessel associated cells in back skin was approximately 20-30%.
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