About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:148693 Narimatsu M, et al., Regulation of planar cell polarity by Smurf ubiquitin ligases. Cell. 2009 Apr 17;137(2):295-307
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7327067
Gene symbol: Prickle1
Gene name: prickle planar cell polarity protein 1
Results
Specimen 6A WT: embryonic day 18.5 (more )
Note: Double labeled: white - Prickle1; red - phalloidin.
Structure Level Pattern Image Note
TS26: cochlea Present Regionally restricted 6A WT Strong colocalization with F-actin in crescent on medial aspect, extending apically into cuticular plate.
Specimen 6A Smurf1+/-;Smurf2-/-: embryonic day 18.5; Smurf1tm1Wran/Smurf1+, Smurf2tm1Wran/Smurf2tm1Wran (more )
Note: Double labeled: white - Prickle1; red - phalloidin.
Structure Level Pattern Image Note
TS26: cochlea Present Regionally restricted 6A Smurf1+/-;Smurf2-/- Expansion of expression to lateral surfaces.
Specimen 6B i/ii WT: embryonic day 18.5 (more )
Note: Double labeled: white - Prickle1; red - phalloidin.
Structure Level Pattern Image Note
TS26: cochlea Present Regionally restricted 6Bi WT (right) , 6Bii WT , 6Bi WT (left) No expression at lateral membrane of sensory hair cells.
Specimen 6B i/ii Smurf1+/-;Smurf2-/-: embryonic day 18.5; Smurf1tm1Wran/Smurf1+, Smurf2tm1Wran/Smurf2tm1Wran (more )
Note: Double labeled: white - Prickle1; red - phalloidin.
Structure Level Pattern Image Note
TS26: cochlea Present Regionally restricted 6Bii Smurf1+/-;Smurf2-/- , 6Bi Smurf1+/-;Smurf2-/- (right) , 6Bi Smurf1+/-;Smurf2-/- (left) Accumulation at lateral membrane of sensory hair cells. Expression around entire circumference pf cortical actin.
Specimen 7A Control: embryonic day 8.0 (more )
Note: Double labeled: red - Prickle1; green - Ctnnb1 (assay MGI:7327065).
Structure Level Pattern Image Note
TS12: neural tube floor plate Present Regionally restricted 7A Control (left) , 7A Control (right) Expression in membraine of neuroectoderm cells.
Specimen 7A Smurf1-/-;Smurf2-/-: embryonic day 8.0; Smurf1tm1Wran/Smurf1tm1Wran, Smurf2tm1Wran/Smurf2tm1Wran (more )
Note: Double labeled: red - Prickle1; green - Ctnnb1 (assay MGI:7327065).
Structure Level Pattern Image Note
TS12: neural tube floor plate Present Regionally restricted 7A Smurf1-/-;Smurf2-/- (left) , 7A Smurf1-/-;Smurf2-/- (right) Expression asymmetrically distributed in individual neuroectoderm cells.
Specimen S9 top (upper): embryonic day 8.0 (more )
Note: Double labeled: red - Prickle1; green - Ctnnb1 (assay MGI:7327065).
Structure Level Pattern Image Note
TS12: neural tube floor plate Present S9_top anti-Prickle1 (upper) , S9_top Merged (upper) , S9_top Merged (enlarged) Specific expression in neurectoderm cells.
Specimen S9 top (lower): embryonic day 8.0 (more )
Note: Double labeled: red - Prickle1; green - Ctnnb1 (assay MGI:7327065).
Structure Level Pattern Image Note
TS12: neural tube floor plate Present S9_top Merged (lower) , S9_top anti-Prickle1 (lower) Specific expression in neurectoderm cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory