Ddx4 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:133516 Alton M, et al., The behavior of the X- and Y-chromosomes in the oocyte during meiotic prophase in the B6.Y(TIR)sex-reversed mouse ovary. Reproduction. 2008 Feb;135(2):241-52
Assay type:	Western blot
MGI Accession ID:	MGI:7314920
Gene symbol:	Ddx4
Gene name:	DEAD box helicase 4

Antibody:	anti-Mvh
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Image: 9MVH

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	88 kDa (a)	Genetic Background	Mutant Allele(s)	Sex	Note
14.5 XX	E14.5	TS22: ovary	Present	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1		Female
14.5 XY	E14.5	TS22: ovary	Present	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1	X/Sry^POS-TIR	Not Specified	sex reversed
17.5 XX	E17.5	TS25: ovary	Present	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1		Female
17.5 XY	E17.5	TS25: ovary	Present	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1	X/Sry^POS-TIR	Not Specified	sex reversed
20.5 dpc XX	P newborn	TS27: ovary	Present	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1		Female
20.5 dpc XY	P newborn	TS27: ovary	Weak	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1	X/Sry^POS-TIR	Not Specified	sex reversed

Notes:
(a) The band intensity in the XY ovary was higher than that in the XX ovary at 14.5 and 17.5 dpc although significant difference was found only at 17.5 dpc. The band in the XY ovary was considerably fainter than that in the XX ovary at 20.5 dpc in two out of three experiments, but no significant difference was found.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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