Gng3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:74178 Schuller U, et al., Developmental expression of heterotrimeric G-proteins in the murine cerebellar cortex. Histochem Cell Biol. 2001 Aug;116(2):149-59
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7314906
Gene symbol:	Gng3
Gene name:	guanine nucleotide binding protein (G protein), gamma 3

Antibody:	anti-Gng3 (sc-375)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Results

Specimen 2L: postnatal day 9 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	2L	Expression is in an ill-defined area surrounding the Purkinje cell bodies.

Specimen 2M: postnatal day 15 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Weak	Regionally restricted	2M	Expression is weak in dendrites and is absent in soma.
TS28: cerebellum internal granule cell layer		Present		2M	Expression products are concentrated withing glomeruli.
TS28: cerebellum molecular layer		Present	Regionally restricted	2M	Expression is in granule cell axons.

Specimen 2N: postnatal adult (more

)

Structure	Level	Image	Note
TS28: cerebellum internal granule cell layer	Present	2N	Expression products are concentrated withing glomeruli.
TS28: cerebellum molecular layer	Present	2N	Expression is in granule cell axons.
TS28: cerebellum Purkinje cell layer	Absent	2N

Specimen Table 3 P3: postnatal day 3 (more

)

Structure	Cell Type	Level	Note
TS27: cerebellum Purkinje cell layer	Purkinje cell	Moderate	Expression is moderate in the soma and dendrites.
TS27: cerebellum external granule cell layer		Absent
TS27: cerebellum internal granule cell layer		Absent

Specimen Table 3 P5: postnatal day 5 (more

)

Structure	Cell Type	Level	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Moderate	Expression is moderate in the soma and dendrites.
TS28: cerebellum external granule cell layer		Absent
TS28: cerebellum internal granule cell layer		Absent

Specimen Table 3 P8: postnatal day 8 (more

)

Structure	Cell Type	Level	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Moderate	Expression is moderate in the soma and dendrites.
TS28: cerebellum internal granule cell layer		Weak
TS28: cerebellum external granule cell layer		Absent

Specimen Table 3 P15: postnatal day 15 (more

)

Structure	Cell Type	Level	Note
TS28: cerebellum internal granule cell layer		Moderate
TS28: cerebellum molecular layer		Moderate
TS28: cerebellum Purkinje cell layer	Purkinje cell	Weak	Expression is weak in dendrites and is absent in soma.

Specimen Table 3 Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Moderate
TS28: cerebellum molecular layer	Moderate
TS28: cerebellum Purkinje cell layer	Absent

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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