Gnai2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:74178 Schuller U, et al., Developmental expression of heterotrimeric G-proteins in the murine cerebellar cortex. Histochem Cell Biol. 2001 Aug;116(2):149-59
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7314904
Gene symbol:	Gnai2
Gene name:	G protein subunit alpha i2

Antibody:	anti-Gnai2 (sc-7276)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Results

Specimen 2E: postnatal day 8 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		2E	Expression is moderate in the soma and the proximal dendrites.
TS28: cerebellum external granule cell layer		Present	Regionally restricted	2E	Expression is in the outer proliferative part.
TS28: cerebellum internal granule cell layer		Weak		2E	There is a discrepancy between Table 3 and the figure legend. The table indicates expression is absent whereas the legend of Figure 2E states there is weak expression.

Specimen Table 3 P3: postnatal day 3 (more

)

Structure	Cell Type	Level	Pattern	Note
TS27: cerebellum Purkinje cell layer	Purkinje cell	Moderate		Expression is moderate in the soma and absent in dendrites.
TS27: cerebellum external granule cell layer		Weak	Regionally restricted	Expression is weak in the outer proliferative part and is absent in the inner premigratory part of the external granule cell layer.
TS27: cerebellum internal granule cell layer		Absent

Specimen Table 3 P5: postnatal day 5 (more

)

Structure	Cell Type	Level	Pattern	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Moderate		Expression is moderate in the soma and absent in dendrites.
TS28: cerebellum external granule cell layer		Weak	Regionally restricted	Expression is weak in the outer proliferative part and is absent in the inner premigratory part of the external granule cell layer.
TS28: cerebellum internal granule cell layer		Absent

Specimen Table 3 P8: postnatal day 8 (more

)

Structure	Cell Type	Level	Pattern	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Moderate		Expression is moderate in the soma and proximal dendrites.
TS28: cerebellum external granule cell layer		Weak	Regionally restricted	Expression is weak in the outer proliferative part and is absent in the inner premigratory part of the external granule cell layer.
TS28: cerebellum internal granule cell layer		Absent		There is a discrepancy between Table 3 and the figure legend. The table indicates expression is absent whereas the legend of Figure 2E states there is weak expression.

Specimen Table 3 P15: postnatal day 15 (more

)

Structure	Cell Type	Level	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Moderate	Expression is moderate in the soma and absent in dendrites.
TS28: cerebellum internal granule cell layer		Absent
TS28: cerebellum molecular layer		Absent

Specimen Table 3 Adult: postnatal adult (more

)

Structure	Cell Type	Level	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Moderate	Expression is moderate in the soma and absent in dendrites.
TS28: cerebellum internal granule cell layer		Weak
TS28: cerebellum molecular layer		Weak

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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