Gene Expression Data
Assay Details
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Assay
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Reference:
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J:133516
Alton M, et al., The behavior of the X- and Y-chromosomes in the oocyte during meiotic prophase in the B6.Y(TIR)sex-reversed mouse ovary. Reproduction. 2008 Feb;135(2):241-52
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7314841
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Gene symbol:
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Ddx4
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Gene name:
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DEAD box helicase 4
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Antibody:
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anti-Mvh
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Fixative: a fresh mixture of absolute ethanol and acetic acid at 3:1 ratio.
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Results
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Specimen
2C XX:
postnatal newborn
(more  )
Note: 24.5 dpc
Specimen
2C XX:
(close  )
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Genetic Background:
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(C57BL/6J x C57BL/6-Chr YTirano/Na)F1
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Age:
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postnatal newborn
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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24.5 dpc
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of ovary |
Present |
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2C
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Numerous cells with cytoplasmic staining were detected.
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Specimen
2D XY:
postnatal newborn; X/ SryPOS-TIR
(more )
Note: 24.5 dpc; sex reversed
Specimen
2D XY:
(close )
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Genetic Background:
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(C57BL/6J x C57BL/6-Chr YTirano/Na)F1
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Mutant Allele(s):
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X/SryPOS-TIR
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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24.5 dpc; sex reversed
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Specimen
3E XX:
postnatal newborn; Paf/Paf +
(more )
Note: 24.5 dpc
Specimen
3E XX:
(close )
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Genetic Background:
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B6.C3Sn-Paf
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Mutant Allele(s):
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Paf/Paf+
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Age:
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postnatal newborn
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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24.5 dpc
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of ovary |
Present |
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3E
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Numerous germ cells were seen throughout the ovary.
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Specimen
3F XO (maternal X):
postnatal newborn; Paf +/?
(more )
Note: 24.5 dpc
Specimen
3F XO (maternal X):
(close )
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Genetic Background:
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B6.C3Sn-Paf
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Mutant Allele(s):
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Paf+/?
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Age:
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postnatal newborn
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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24.5 dpc
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of ovary |
Present |
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3F
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Numerous germ cells were seen throughout the ovary.
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Analysis Tools
