Gene Expression Data
Assay Details
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Assay
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Reference:
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J:133516
Alton M, et al., The behavior of the X- and Y-chromosomes in the oocyte during meiotic prophase in the B6.Y(TIR)sex-reversed mouse ovary. Reproduction. 2008 Feb;135(2):241-52
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7314832
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Gene symbol:
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Gcna
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Gene name:
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germ cell nuclear acidic protein
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Antibody:
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anti-GCNA (10D9G11)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Fixative: a fresh mixture of absolute ethanol and acetic acid at 3:1 ratio.
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Results
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Specimen
1A XX:
embryonic day 14.5
(more  )
Specimen
1A XX:
(close  )
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Genetic Background:
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(C57BL/6J x C57BL/6-Chr YTirano/Na)F1
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Age:
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embryonic day 14.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: germ cell of ovary |
Present |
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1A
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Germ cells were distributed throughout the ovary.
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Specimen
1B XY:
embryonic day 14.5; X/ SryPOS-TIR
(more )
Note: sex reversed
Specimen
1B XY:
(close )
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Genetic Background:
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(C57BL/6J x C57BL/6-Chr YTirano/Na)F1
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Mutant Allele(s):
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X/SryPOS-TIR
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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sex reversed
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Specimen
1C XX:
embryonic day 15.5
(more )
Specimen
1C XX:
(close )
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Genetic Background:
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(C57BL/6J x C57BL/6-Chr YTirano/Na)F1
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Age:
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embryonic day 15.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: germ cell of ovary |
Present |
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1C
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Germ cells were distributed throughout the ovary.
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Specimen
1D XY:
embryonic day 15.5; X/ SryPOS-TIR
(more )
Note: sex reversed
Specimen
1D XY:
(close )
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Genetic Background:
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(C57BL/6J x C57BL/6-Chr YTirano/Na)F1
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Mutant Allele(s):
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X/SryPOS-TIR
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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sex reversed
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Specimen
1E XX:
embryonic day 17.5
(more )
Specimen
1E XX:
(close )
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Genetic Background:
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(C57BL/6J x C57BL/6-Chr YTirano/Na)F1
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Age:
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embryonic day 17.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: germ cell of ovary |
Present |
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1E
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Germ cells were distributed throughout the ovary.
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Specimen
1F XY:
embryonic day 17.5; X/ SryPOS-TIR
(more )
Note: sex reversed
Specimen
1F XY:
(close )
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Genetic Background:
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(C57BL/6J x C57BL/6-Chr YTirano/Na)F1
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Mutant Allele(s):
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X/SryPOS-TIR
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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sex reversed
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: germ cell of ovary |
Present |
Regionally restricted |
1F
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The intensity of immunostaining was comparable between XY and XX ovaries. However, considerably fewer germ cells were distributed in the central region of the XY ovary.
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Specimen
1G XX:
embryonic day 19.5
(more )
Specimen
1G XX:
(close )
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Genetic Background:
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(C57BL/6J x C57BL/6-Chr YTirano/Na)F1
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Age:
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embryonic day 19.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: germ cell of ovary |
Present |
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1G
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Fewer germ cells were noted in the XX ovary compared with earlier ages.
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Specimen
1H XY:
embryonic day 19.5; X/ SryPOS-TIR
(more )
Note: sex reversed
Specimen
1H XY:
(close )
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Genetic Background:
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(C57BL/6J x C57BL/6-Chr YTirano/Na)F1
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Mutant Allele(s):
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X/SryPOS-TIR
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Age:
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embryonic day 19.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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sex reversed
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: germ cell of ovary |
Present |
Regionally restricted |
1H
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Fewer germ cells were seen in the XY ovary compared with earlier ages. In particular, germ cells were scarce in the central region of the XY ovary.
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Specimen
1I XX:
postnatal newborn
(more )
Note: 21.5 dpc
Specimen
1I XX:
(close )
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Genetic Background:
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(C57BL/6J x C57BL/6-Chr YTirano/Na)F1
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Age:
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postnatal newborn
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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21.5 dpc
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of ovary |
Present |
Non-Uniform |
1I
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Germ cells were seen in the entire region of the XX ovary, although a larger number were seen in the peripheral region.
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Specimen
1J XY:
postnatal newborn; X/ SryPOS-TIR
(more )
Note: 21.5 dpc; sex reversed
Specimen
1J XY:
(close )
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Genetic Background:
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(C57BL/6J x C57BL/6-Chr YTirano/Na)F1
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Mutant Allele(s):
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X/SryPOS-TIR
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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21.5 dpc; sex reversed
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of ovary |
Weak |
Regionally restricted |
1J
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Fewer germ cells were seen in the XY ovary, mainly in the peripheral region.
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Specimen
2A XX:
postnatal newborn
(more )
Note: 24.5 dpc
Specimen
2A XX:
(close )
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Genetic Background:
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(C57BL/6J x C57BL/6-Chr YTirano/Na)F1
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Age:
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postnatal newborn
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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24.5 dpc
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of ovary |
Weak |
Single cells |
2A
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A few germ cells with faint immunostaining were seen in the peripheral region of the XX ovary.
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Specimen
2B XY:
postnatal newborn; X/ SryPOS-TIR
(more )
Note: 24.5 dpc; sex reversed
Specimen
2B XY:
(close )
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Genetic Background:
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(C57BL/6J x C57BL/6-Chr YTirano/Na)F1
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Mutant Allele(s):
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X/SryPOS-TIR
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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24.5 dpc; sex reversed
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of ovary |
Trace |
Regionally restricted |
2B
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Staining is almost absent in the XY ovary.
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Specimen
3A XX:
embryonic day 15.5; Paf/Paf +
(more )
Specimen
3A XX:
(close )
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Genetic Background:
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B6.C3Sn-Paf
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Mutant Allele(s):
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Paf/Paf+
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Age:
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embryonic day 15.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: germ cell of ovary |
Present |
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3A
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Numerous germ cells were seen throughout the ovary.
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Specimen
3B XO (maternal X):
embryonic day 15.5; Paf +/?
(more )
Specimen
3B XO (maternal X):
(close )
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Genetic Background:
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B6.C3Sn-Paf
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Mutant Allele(s):
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Paf+/?
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Age:
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embryonic day 15.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: germ cell of ovary |
Present |
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3B
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Numerous germ cells were seen throughout the ovary.
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Specimen
3C XX:
postnatal newborn; Paf/Paf +
(more )
Note: 20.5 dpc
Specimen
3C XX:
(close )
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Genetic Background:
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B6.C3Sn-Paf
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Mutant Allele(s):
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Paf/Paf+
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Age:
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postnatal newborn
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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20.5 dpc
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of ovary |
Present |
Regionally restricted |
3C
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Fewer germ cells were seen, particularly in the central region compared with E15.5.
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Specimen
3D XO (maternal X):
postnatal newborn; Paf +/?
(more )
Note: 20.5 dpc
Specimen
3D XO (maternal X):
(close )
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Genetic Background:
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B6.C3Sn-Paf
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Mutant Allele(s):
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Paf+/?
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Age:
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postnatal newborn
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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20.5 dpc
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of ovary |
Present |
Regionally restricted |
3D
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Fewer germ cells were seen, particularly in the central region compared with E15.5.
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