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Gene Expression Data
Assay Details
Assay
Reference: J:321931 Liu J, et al., Molecular divergence of mammalian astrocyte progenitor cells at early gliogenesis. Development. 2022 Mar 1;149(5):dev199985
Assay type: RNA in situ
MGI Accession ID: MGI:7312037
Gene symbol: Sparcl1
Gene name: SPARC-like 1
Probe: Mm-Sparcl1 (424641)
Probe preparation: Antisense
Visualized with: Fluorescence
Assay notes: RNAscope probe. Amplification channel C1.
Results
Specimen 3A (upper): embryonic day 16.5 (more )
Note: Triple labeled: red - Sparcl1; green - Sparc (assay MGI:7312036); purple - Pecam1 immunohistochemistry (assay MGI:7312028).
Structure Level Pattern Image Note
TS24: cerebral cortex subventricular zone Present Regionally restricted 3A Sparcl1 (upper) , 3A Merge (upper) Expression in dorsolateral subventricular zone region.
TS24: brain blood vessel Present 3A Sparcl1 (upper) , 3A Merge (upper)
Specimen 3A (lower): embryonic day 16.5 (more )
Note: Triple labeled: red - Sparcl1; green - Sparc (assay MGI:7312036); purple - Pecam1 immunohistochemistry (assay MGI:7312028).
Structure Level Pattern Image Note
TS24: cerebral cortex Present Regionally restricted 3A Merge (lower) , 3A Sparcl1 (lower) Expression gradually extended into dorsal cortex.
TS24: brain blood vessel Present 3A Merge (lower) , 3A Sparcl1 (lower)
Specimen 3B: embryonic day 18.5 (more )
Note: Triple labeled: red - Sparcl1; green - Sparc (assay MGI:7312036); purple - Pecam1 immunohistochemistry (assay MGI:7312028).
Structure Level Pattern Image Note
TS26: cerebral cortex Present Regionally restricted 3B Merge , 3B Sparcl1 Expression gradually extended into dorsal cortex.
TS26: brain blood vessel Present 3B Sparcl1 , 3B Merge
Specimen 3C: embryonic day 18.5 (more )
Note: Triple labeled: red - Sparcl1; green - Sparc (assay MGI:7312036); purple - Pecam1 immunohistochemistry (assay MGI:7312028).
Structure Level Pattern Image Note
TS26: telencephalon Present Regionally restricted 3C Expression in cells intermixed with Sparc-expressing cells.
TS26: brain blood vessel Present 3C
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory