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Gene Expression Data
Assay Details
Assay
Reference: J:321931 Liu J, et al., Molecular divergence of mammalian astrocyte progenitor cells at early gliogenesis. Development. 2022 Mar 1;149(5):dev199985
Assay type: RNA in situ
MGI Accession ID: MGI:7312036
Gene symbol: Sparc
Gene name: secreted acidic cysteine rich glycoprotein
Probe: Mm-Sparc (466781)
Probe preparation: Antisense
Visualized with: Fluorescence
Assay notes: RNAscope probe. Amplification channel C2.
Results
Specimen 3A (upper): embryonic day 16.5 (more )
Note: Triple labeled: green - Sparc; red - Sparcl1 (assay MGI:7312037); purple - Pecam1 immunohistochemistry (assay MGI:7312028).
Structure Level Pattern Image Note
TS24: telencephalon ventricular layer Present 3A Merge (upper) , 3A Sparc (upper) Expression along ventricular zone.
TS24: brain blood vessel Present 3A Sparc (upper) , 3A Merge (upper)
Specimen 3A (lower): embryonic day 16.5 (more )
Note: Triple labeled: green - Sparc; red - Sparcl1 (assay MGI:7312037); purple - Pecam1 immunohistochemistry (assay MGI:7312028).
Structure Level Pattern Image Note
TS24: telencephalon ventricular layer Present 3A Sparc (lower) , 3A Merge (lower) Expression along ventricular zone.
TS24: brain blood vessel Present 3A Merge (lower) , 3A Sparc (lower)
Specimen 3B: embryonic day 18.5 (more )
Note: Triple labeled: green - Sparc; red - Sparcl1 (assay MGI:7312037); purple - Pecam1 immunohistochemistry (assay MGI:7312028).
Structure Level Pattern Image Note
TS26: telencephalon ventricular layer Present 3B Merge , 3B Sparc Expression along ventricular zone.
TS26: brain blood vessel Present 3B Sparc , 3B Merge
Specimen 3C: embryonic day 18.5 (more )
Note: Triple labeled: green - Sparc; red - Sparcl1 (assay MGI:7312037); purple - Pecam1 immunohistochemistry (assay MGI:7312028).
Structure Level Pattern Image Note
TS26: telencephalon Present Regionally restricted 3C Expression in cells intermixed with Sparcl1-expressing cells.
TS26: brain blood vessel Present 3C
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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