Sparcl1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:321931 Liu J, et al., Molecular divergence of mammalian astrocyte progenitor cells at early gliogenesis. Development. 2022 Mar 1;149(5):dev199985
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7312032
Gene symbol:	Sparcl1
Gene name:	SPARC-like 1

Antibody:	anti-SPARCL1 (AF2836)
Detection system:	Secondary antibody

Results

S8B ©

Specimen S8B: embryonic day 17.5 (more

)
Note: Double labeled: green - Sparcl1; red - Mki67 (assay MGI:7312035).

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present	Regionally restricted	S8B	Expression in non-blood vessel cells beneath pallium-subpallium junction.

Specimen S8B inset: embryonic day 17.5 (more

)
Note: Double labeled: green - Sparcl1; red - Mki67 (assay MGI:7312035).

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present	Regionally restricted	S8B inset right , S8B inset middle	Expression in cells originating at septum-subpallium junction.

S8D1 Merge ©

S8D1 SPARCL1 ©

Specimen S8D1: embryonic day 17.5 (more

)
Note: Double labeled: green - Sparcl1; red - Mki67 (assay MGI:7312035).

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present	Regionally restricted	S8D1 SPARCL1 , S8D1 Merge	Expression in dividing cells.

S8D2 Merge ©

S8D2 SPARCL1 ©

Specimen S8D2: embryonic day 17.5 (more

)
Note: Double labeled: green - Sparcl1; red - Mki67 (assay MGI:7312035).

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present	Regionally restricted	S8D2 Merge , S8D2 SPARCL1	Expression in dividing cells.

S8D3 Merge ©

S8D3 SPARCL1 ©

Specimen S8D3: embryonic day 17.5 (more

)
Note: Double labeled: green - Sparcl1; red - Mki67 (assay MGI:7312035).

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present	Regionally restricted	S8D3 SPARCL1 , S8D3 Merge	Expression in dividing cells.

S8D4 Merge ©

S8D4 SPARCL1 ©

Specimen S8D4: embryonic day 17.5 (more

)
Note: Double labeled: green - Sparcl1; red - Mki67 (assay MGI:7312035).

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present	Regionally restricted	S8D4 Merge , S8D4 SPARCL1	Expression in dividing cells.

Specimen S9 E17.5: embryonic day 17.5 (more

)
Note: Double labeled: green - Sparcl1; red - Mki67 (assay MGI:7312035).

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present	Regionally restricted	S9_E17.5 SPARCL1 , S9_E17.5 (merge)	Prominent expression in astrocyte progenitor cells (APCs) in ventral side of pallium-subpallium boundary (PSB). Expression in some cells appearing to stream out towards lateral-ventral areas - likely differentiating astrocytes originating from PSB.

Specimen S9 E19.5: embryonic day 19.5 (more

)
Note: Double labeled: green - Sparcl1; red - Mki67 (assay MGI:7312035).

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Present	Regionally restricted	S9_E19.5 SPARCL1 , S9_E19.5 (merge)	Expression in astrocyte progenitor cells (APCs) mainly in dorsal side of pallium-subpallium boundary (PSB), distributing medially into cortex proper.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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