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Gene Expression Data
Assay Details
Assay
Reference: J:321931 Liu J, et al., Molecular divergence of mammalian astrocyte progenitor cells at early gliogenesis. Development. 2022 Mar 1;149(5):dev199985
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7312026
Gene symbol: Sparc
Gene name: secreted acidic cysteine rich glycoprotein
Antibody: anti-SPARC (AF942)
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 2A E16.5: embryonic day 16.5 (more )
Note: Double labeled: red - Sparc; green - Pecam1 (assay MGI:7312027).
Structure Level Pattern Image Note
TS24: telencephalon ventricular layer Strong Regionally restricted 2A E16.5 (merge) , 2A E16.5 SPARC Prominent expression in cells lining lateral ventricular zone.
TS24: brain blood vessel Present 2A E16.5 (merge) , 2A E16.5 SPARC
Specimen 2A E18.5: embryonic day 18.5 (more )
Note: Double labeled: red - Sparc; green - Pecam1 (assay MGI:7312027).
Structure Level Pattern Image Note
TS26: telencephalon ventricular layer Strong Regionally restricted 2A E18.5 (merge) , 2A E18.5 SPARC Prominent expression in cells lining lateral ventricular zone.
TS26: brain blood vessel Present 2A E18.5 (merge) , 2A E18.5 SPARC
Specimen 2A P1: postnatal day 1 (more )
Note: Double labeled: red - Sparc; green - Pecam1 (assay MGI:7312027).
Structure Level Pattern Image Note
TS27: cerebral cortex ventricular zone Strong Regionally restricted 2A P1 SPARC , 2A P1 (merge) Prominent expression in cells lining lateral ventricular zone.
TS27: brain blood vessel Present 2A P1 (merge) , 2A P1 SPARC
Specimen 2B: postnatal day 1 (more )
Note: Double labeled: red - Sparc; green - Pecam1 (assay MGI:7312027).
Structure Level Pattern Image Note
TS27: cerebral cortex Present Regionally restricted 2B SPARC , 2B (merge) Expression in cell processes extending to cortical pial surface.
TS27: brain blood vessel Present 2B (merge) , 2B SPARC
Specimen S5A E16.5: embryonic day 16.5 (more )
Note: Double labeled: red - Sparc; green - Pecam1 (assay MGI:7312027).
Structure Level Pattern Image Note
TS24: ganglionic eminence Present Regionally restricted S5A E16.5 (merge) , S5A E16.5 SPARC Expression in radial processes associated with ventricular astrocyte progenitor cells (APCs) in ganglionic eminence regions.
TS24: brain blood vessel Present S5A E16.5 (merge) , S5A E16.5 SPARC
Specimen S5A E18.5: embryonic day 18.5 (more )
Note: Double labeled: red - Sparc; green - Pecam1 (assay MGI:7312027).
Structure Level Pattern Image Note
TS26: ganglionic eminence Present Regionally restricted S5A E18.5 (merge) , S5A E18.5 SPARC Expression in radial processes associated with ventricular astrocyte progenitor cells (APCs) in ganglionic eminence regions.
TS26: brain blood vessel Present S5A E18.5 SPARC , S5A E18.5 (merge)
Specimen S5A P1: postnatal day 1 (more )
Note: Double labeled: red - Sparc; green - Pecam1 (assay MGI:7312027).
Structure Level Pattern Image Note
TS27: ganglionic eminence Present Regionally restricted S5A P1 (merge) , S5A P1 SPARC Expression in radial processes associated with ventricular astrocyte progenitor cells (APCs) in ganglionic eminence regions.
TS27: brain blood vessel Present S5A P1 (merge) , S5A P1 SPARC
Specimen S5B E17.5: embryonic day 17.5 (more )
Note: Double labeled: red - Sparc; green - Pecam1 (assay MGI:7312027).
Structure Level Pattern Image Note
TS25: telencephalon ventricular layer Present Regionally restricted S5B E17.5 Merge , S5B E17.5 SPARC Expression in cells lining ventricles - astrocyte progenitor cells (APCs).
TS25: telencephalon Present Regionally restricted S5B E17.5 Merge , S5B E17.5 SPARC Expression in cells outside of ventricular zone - presumably differentiating astrocytes.
TS25: brain blood vessel Present S5B E17.5 Merge , S5B E17.5 SPARC
Specimen S5B E18.5: embryonic day 18.5 (more )
Note: Double labeled: red - Sparc; green - Pecam1 (assay MGI:7312027).
Structure Level Pattern Image Note
TS26: telencephalon ventricular layer Present Regionally restricted S5B E18.5 SPARC , S5B E18.5 Merge Expression in cells lining ventricles - astrocyte progenitor cells (APCs).
TS26: brain blood vessel Present S5B E18.5 Merge , S5B E18.5 SPARC
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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