Gene Expression Data
Assay Details
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Assay
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Reference:
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J:326256
Lee JM, et al., MSX1 Drives Tooth Morphogenesis Through Controlling Wnt Signaling Activity. J Dent Res. 2022 Jul;101(7):832-839
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7311642
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Gene symbol:
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Lef1
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Gene name:
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lymphoid enhancer binding factor 1
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Probe:
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Lef1 probe19
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Probe preparation:
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labelled with digoxigenin
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A Mx:
embryonic day 12.5
(more  )
Specimen
1A Mx:
(close  )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: dental placode |
Strong |
Regionally restricted |
1A Mx
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Strong expression in embryonic tooth germ, concentrated in distal tip.
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Specimen
1A' Mx:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Msx1tm1Rilm/Msx1tm1Rilm
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: dental placode |
Weak |
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1A' Mx
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Expression markedly reduced compared with controls.
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Specimen
1A Mn:
embryonic day 12.5
(more )
Specimen
1A Mn:
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: dental placode |
Strong |
Regionally restricted |
1A Mn
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Strong expression in embryonic tooth germ, concentrated in distal tip.
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Specimen
1A' Mn:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Msx1tm1Rilm/Msx1tm1Rilm
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: dental placode |
Weak |
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1A' Mn
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Expression markedly reduced compared with controls.
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Specimen
1B Mx:
embryonic day 13.5
(more )
Specimen
1B Mx:
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: tooth bud |
Strong |
Regionally restricted |
1B Mx
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Strong expression in embryonic tooth germ, concentrated in distal tip.
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Specimen
1B' Mx:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Msx1tm1Rilm/Msx1tm1Rilm
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: tooth bud |
Weak |
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1B' Mx
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Expression markedly reduced compared with controls.
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Specimen
1B Mn:
embryonic day 13.5
(more )
Specimen
1B Mn:
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: tooth bud |
Strong |
Regionally restricted |
1B Mn
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Strong expression in embryonic tooth germ, concentrated in distal tip.
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Specimen
1B' Mn:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Msx1tm1Rilm/Msx1tm1Rilm
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: tooth bud |
Weak |
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1B' Mn
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Expression markedly reduced compared with controls.
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Specimen
1C Mx:
embryonic day 14.5
(more )
Specimen
1C Mx:
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cap stage tooth |
Strong |
Regionally restricted |
1C Mx
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Strong expression in embryonic tooth germ, concentrated in distal tip.
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Specimen
1C' Mx:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Msx1tm1Rilm/Msx1tm1Rilm
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cap stage tooth |
Weak |
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1C' Mx
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Expression markedly reduced compared with controls.
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Specimen
1C Mn:
embryonic day 14.5
(more )
Specimen
1C Mn:
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cap stage tooth |
Strong |
Regionally restricted |
1C Mn
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Strong expression in embryonic tooth germ, concentrated in distal tip.
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Specimen
1C' Mn:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Msx1tm1Rilm/Msx1tm1Rilm
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cap stage tooth |
Weak |
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1C' Mn
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Expression markedly reduced compared with controls.
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Specimen
3J (upper):
embryonic day 14.0
(more )
Specimen
3J (upper):
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3K (upper):
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Msx1tm1Rilm/Msx1tm1Rilm
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: upper jaw molar |
Weak |
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3K (upper)
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Marked reduction in expression compared with control. Residual expression higher in maxilla than in mandible.
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Specimen
3L (upper):
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: upper jaw molar |
Present |
Regionally restricted |
3L (upper)
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Comsiderable restoration of expression in dental epithelium and mesenchyme.
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Specimen
3J (lower):
embryonic day 14.0
(more )
Specimen
3J (lower):
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3K (lower):
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Msx1tm1Rilm/Msx1tm1Rilm
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3L (lower):
(close )
Specimen
4H (upper):
embryonic day 14.25
(more )
Specimen
4H (upper):
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 14.25
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4I (upper):
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Msx1tm1Rilm/Msx1tm1Rilm
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Age:
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embryonic day 14.25
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: upper jaw molar |
Weak |
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4I (upper)
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Markedly reduced expression compared with control.
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Specimen
4J (upper):
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: upper jaw molar |
Strong |
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4J (upper)
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Considerable recovery of expression in maxillary molar.
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Specimen
4H (lower):
embryonic day 14.25
(more )
Specimen
4H (lower):
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 14.25
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4I (lower):
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Msx1tm1Rilm/Msx1tm1Rilm
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Age:
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embryonic day 14.25
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lower jaw molar |
Weak |
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4I (lower)
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Markedly reduced expression compared with control.
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Specimen
4J (lower):
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lower jaw molar |
Present |
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4J (lower)
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Minor recovery of expression in mandibular molar.
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