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Gene Expression Data
Assay Details
Assay
Reference: J:324126 Hiltunen AE, et al., Nhlrc2 is crucial during mouse gastrulation. Genesis. 2022 Mar;60(3):e23470
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7309975
Gene symbol: Nhlrc2
Gene name: NHL repeat containing 2
Reporter: lacZ
Direct Detection
Results
Specimen 5a E6.5 untreated top: embryonic day 6.5; Nhlrc2tm1a(KOMP)Wtsi/Nhlrc2+ (more )
Note: Post-fixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS9: embryo Strong Regionally restricted 5a E6.5 het top left Strongest staining was in the epiblast and ectoderm layers.
TS9: embryo ectoderm Strong 5a E6.5 het top left
TS9: extraembryonic ectoderm Strong 5a E6.5 het top left
Specimen 5a E6.5 untreated bottom: embryonic day 6.5; Nhlrc2tm1a(KOMP)Wtsi/Nhlrc2+ (more )
Note: Post-fixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS9: embryo Strong Regionally restricted 5a E6.5 het bottom left Strongest staining was in the epiblast and ectoderm layers.
TS9: embryo ectoderm Strong 5a E6.5 het bottom left
TS9: extraembryonic ectoderm Strong 5a E6.5 het bottom left
Specimen 5a E6.5 BABB cleared top: embryonic day 6.5; Nhlrc2tm1a(KOMP)Wtsi/Nhlrc2+ (more )
Note: Embryo clearing was performed using 1:2 benzoic alcohol-benzyl benzoate (BABB). Post-fixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS9: embryo Strong Regionally restricted 5a E6.5 het top right Strongest staining was in the epiblast and ectoderm layers.
TS9: embryo ectoderm Strong 5a E6.5 het top right
TS9: extraembryonic ectoderm Strong 5a E6.5 het top right
Specimen 5a E6.5 BABB cleared bottom: embryonic day 6.5; Nhlrc2tm1a(KOMP)Wtsi/Nhlrc2+ (more )
Note: Embryo clearing was performed using 1:2 benzoic alcohol-benzyl benzoate (BABB). Post-fixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS9: embryo Strong Regionally restricted 5a E6.5 het bottom right Strongest staining was in the epiblast and ectoderm layers.
TS9: embryo ectoderm Strong 5a E6.5 het bottom right
TS9: extraembryonic ectoderm Strong 5a E6.5 het bottom right
Specimen 5a E7.5 untreated top: embryonic day 7.5; Nhlrc2tm1a(KOMP)Wtsi/Nhlrc2+ (more )
Note: Post-fixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS11: embryo Strong Regionally restricted 5a E7.5 het top left Strongest staining was in the epiblast and ectoderm layers.
TS11: allantois Strong 5a E7.5 het top left
TS11: amnion Strong 5a E7.5 het top left
TS11: embryo ectoderm Strong 5a E7.5 het top left
TS11: extraembryonic ectoderm Strong 5a E7.5 het top left
Specimen 5a E7.5 untreated bottom: embryonic day 7.5; Nhlrc2tm1a(KOMP)Wtsi/Nhlrc2+ (more )
Note: Post-fixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS11: embryo Strong Regionally restricted 5a E7.5 het bottom left Strongest staining was in the epiblast and ectoderm layers.
TS11: allantois Strong 5a E7.5 het bottom left
TS11: amnion Strong 5a E7.5 het bottom left
TS11: embryo ectoderm Strong 5a E7.5 het bottom left
TS11: extraembryonic ectoderm Strong 5a E7.5 het bottom left
Specimen 5a E7.5 BABB cleared top: embryonic day 7.5; Nhlrc2tm1a(KOMP)Wtsi/Nhlrc2+ (more )
Note: Embryo clearing was performed using 1:2 benzoic alcohol-benzyl benzoate (BABB). Post-fixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS11: embryo Strong Regionally restricted 5a E7.5 het top right Strongest staining was in the epiblast and ectoderm layers.
TS11: allantois Strong 5a E7.5 het top right
TS11: amnion Strong 5a E7.5 het top right
TS11: embryo ectoderm Strong 5a E7.5 het top right
TS11: extraembryonic ectoderm Strong 5a E7.5 het top right
Specimen 5a E7.5 BABB cleared bottom: embryonic day 7.5; Nhlrc2tm1a(KOMP)Wtsi/Nhlrc2+ (more )
Note: Embryo clearing was performed using 1:2 benzoic alcohol-benzyl benzoate (BABB). Post-fixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS11: embryo Strong Regionally restricted 5a E7.5 het bottom right Strongest staining was in the epiblast and ectoderm layers.
TS11: allantois Strong 5a E7.5 het bottom right
TS11: amnion Strong 5a E7.5 het bottom right
TS11: embryo ectoderm Strong 5a E7.5 het bottom right
TS11: extraembryonic ectoderm Strong 5a E7.5 het bottom right
Specimen 5b E7.5 left: embryonic day 7.5; Nhlrc2tm1a(KOMP)Wtsi/Nhlrc2+ (more )
Structure Level Pattern Image Note
TS11: allantois Strong 5b E7.5 left
TS11: amnion Strong 5b E7.5 left
TS11: embryo ectoderm Strong 5b E7.5 left
TS11: ectoplacental cone Moderate 5b E7.5 left
TS11: embryo mesoderm Moderate 5b E7.5 left
TS11: definitive endoderm Weak Regionally restricted 5b E7.5 left A small amount of endodermal staining was present, mostly adjacent to the ectoplacental cone.
TS11: embryo endoderm Weak Regionally restricted 5b E7.5 left A small amount of endodermal staining was present, mostly adjacent to the ectoplacental cone.
Specimen 5b E7.5 right: embryonic day 7.5; Nhlrc2tm1a(KOMP)Wtsi/Nhlrc2+ (more )
Structure Level Pattern Image Note
TS11: allantois Strong 5b E7.5 right
TS11: amnion Strong 5b E7.5 right
TS11: embryo ectoderm Strong 5b E7.5 right
TS11: ectoplacental cone Moderate 5b E7.5 right
TS11: embryo mesoderm Moderate 5b E7.5 right
TS11: definitive endoderm Weak Regionally restricted 5b E7.5 right A small amount of endodermal staining was present, mostly adjacent to the ectoplacental cone.
TS11: embryo endoderm Weak Regionally restricted 5b E7.5 right A small amount of endodermal staining was present, mostly adjacent to the ectoplacental cone.
Specimen 5c E7.5 ve, me: embryonic day 7.5; Nhlrc2tm1a(KOMP)Wtsi/Nhlrc2+ (more )
Note: For TEM, embryos were fixed with 1% glutaraldehyde and 4% formaldehyde, post-fixed in 1% osmium tetroxide, and embedded in Epon.
Structure Level Pattern Image Note
TS11: embryo mesoderm Moderate 5c left
TS11: embryonic visceral endoderm Trace 5c left
Specimen 5c E7.5 ec: embryonic day 7.5; Nhlrc2tm1a(KOMP)Wtsi/Nhlrc2+ (more )
Note: For TEM, embryos were fixed with 1% glutaraldehyde and 4% formaldehyde, post-fixed in 1% osmium tetroxide, and embedded in Epon.
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 5c middle
Specimen 5c E7.5 ve, exe: embryonic day 7.5; Nhlrc2tm1a(KOMP)Wtsi/Nhlrc2+ (more )
Note: For TEM, embryos were fixed with 1% glutaraldehyde and 4% formaldehyde, post-fixed in 1% osmium tetroxide, and embedded in Epon.
Structure Level Pattern Image Note
TS11: extraembryonic ectoderm Present 5c right
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory