Probe:	Col2a1 cDNA19
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS20: femur pre-cartilage condensation	Present		2B
TS20: hindlimb pre-cartilage condensation	Present		2B	Expressed in a continuous Y-shaped anlagen (femur+tibia+fibula).

Structure	Level	Pattern	Image	Note
TS21: femur cartilage condensation	Present		2D
TS21: tibia-fibula pre-cartilage condensation	Present		2D
TS21: knee joint primordium	Trace		2D	The interzone had formed and most cells in this region no longer expressed Col2a1.

Structure	Level	Pattern	Image	Note
TS21: knee joint primordium	Trace		5A	No expression in the medial meniscus.
TS21: femur cartilage condensation	Present		5A
TS21: tibia-fibula pre-cartilage condensation	Present		5A

Structure	Level	Pattern	Image	Note
TS22: meniscus of knee	Absent		5B
TS22: femur cartilage condensation	Present		5B
TS22: tibia cartilage condensation	Present		5B

Structure	Level	Pattern	Image	Note
TS23: medial meniscus of knee	Absent		5C
TS23: femur	Present		5C
TS23: tibia	Present		5C

Structure	Level	Pattern	Image	Note
TS27: medial meniscus of knee	Absent		5D
TS27: femur	Present		5D
TS27: tibia	Present		5D

Structure	Level	Pattern	Image	Note
TS28: femur	Present		5E
TS28: medial meniscus of knee	Present		5E
TS28: tibia	Present		5E

Structure	Level	Pattern	Image	Note
TS27: cruciate ligament of knee	Absent		5F
TS27: femur	Present		5F
TS27: tibia	Present		5F

Structure	Level	Pattern	Image	Note
TS21: knee joint primordium	Trace	Regionally restricted		The interzone had formed and most cells in this region no longer expressed Col2a1. No expression in the medial meniscus nor the cruciate ligament cells.

Structure	Level	Pattern	Image	Note
TS22: knee joint primordium	Absent			No expression in the medial meniscus nor the cruciate ligament cells.

Structure	Level	Pattern	Image	Note
TS23: cruciate ligament of knee	Absent

Structure	Level	Pattern	Image	Note
TS28: cruciate ligament of knee	Absent