Probe:	Fzd9 cDNA6
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	1I	Expression is dorsal.
TS15: telencephalon	Present	Regionally restricted	1I	There is a sharp expression border in the dorso-rostral telencephalon.
TS15: head mesenchyme	Present	Regionally restricted	1I	Expression is in the rostral mesenchyme underlying the anterior telencephalon.
TS15: diencephalon	Present	Regionally restricted	1I	Expression is dorsal and in a small domain of the ventral diencephalon.
TS15: future hindbrain	Present		1I

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong	Regionally restricted	2I	Expression is in the dorso-medial telencephalon.
TS17: midbrain	Present	Regionally restricted	2I	Expression is dorsal and in the rostral cephalic flexure across the ventral part of the rostral midbrain.
TS17: diencephalon	Present	Regionally restricted	2I	Expression is dorsal and in a small domain of the ventral diencephalon and in the rostral cephalic flexure across the ventral part of the rostral midbrain and caudal diencephalon.
TS17: hindbrain	Present		2I
TS17: head mesenchyme	Absent		2I

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	3I	Expression is in the dorso-medial telencephalon (including the hippocampal anlage).
TS20: diencephalon	Strong	Regionally restricted	3I	Expression is in the thalamic eminence and is weaker in the preoptic and postoptic area, and in the cephalic flexure. Expression is weak in the subthalamic area, and in the roof of the diencephalon. There is a gap in expression at the caudal part of the cephalic flexure.
TS20: hindbrain	Strong	Regionally restricted	3I	Expression is in the ventral hindbrain up to the midbrain/hindbrain boundary.
TS20: cerebral cortex	Present		3I
TS20: thalamus	Present	Regionally restricted	3I	Expression is in the dorsal thalamus.
TS20: ganglionic eminence	Weak		3I
TS20: metencephalon	Weak		3I
TS20: midbrain roof plate	Weak	Regionally restricted	3I	Expression is in the tectum.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	4G	Expression is along the dorsal and ventral hindbrain.
TS17: midbrain	Present	Regionally restricted	4G	Expression is along the dorsal and ventral midbrain with a domain of weaker expression in the caudal midbrain, both dorsally and ventrally, at the rostral border of the midbrain/hindbrain boundary.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Weak	Regionally restricted	4G'	Expression is in the basal plate up to the alar/basal boundary and in the most dorsal alar plate of the mesencephalon. Expression is absent in the lateral wall (alar plate) and in the dorsal (roof plate) and ventral (medial floor plate) midline of the midbrain. midbrain
TS17: midbrain floor plate	Weak		4G'
TS17: midbrain roof plate	Absent		4G'

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	5H	There is a gap in expression in the caudal cephalic flexure is restricted to the medial floor plate.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Regionally restricted	6H	Expression is strongest in the ventral anterior hindbrain (ventral r1), and is lower in the dorsal anterior hindbrain (dorsal r1) with a sharp rostral border at the midbrain/hindbrain boundary.
TS20: midbrain roof plate	Present	Regionally restricted	6H	Expression is weaker in the caudal tectum.
TS20: midbrain	Strong	Regionally restricted	6H	Expression is in the ventricular zone of the mesencephalic ventral alar plate and basal plate with a sharp ventral border. Expression is weak in the ventricular zone/subventricular zone of the dorsal alar plate and very weak in the cephalic flexure.
TS20: midbrain ventricular layer	Strong	Regionally restricted	6H	Expression is in the ventricular zone of the mesencephalic ventral alar plate and basal plate with a sharp ventral border. Expression is weak in the ventricular zone/subventricular zone of the dorsal alar plate and very weak in the cephalic flexure.

Structure	Level	Pattern	Image	Note
TS20: midbrain floor plate	Absent		6H'

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Graded	8I	Expression is srtong in the hippocampal anlage with a sharp ventral border at the transition to the cortical hem and choroid plexus. Expression is in the dorsomedial telencephalon with a medial-high to lateral-low gradient. Absent in the ventral telencephalon (subpallium).
TS20: diencephalon	Weak	Regionally restricted	8I	Expression is in the thalamic eminence and in a decreasing gradient toward the dorsal thalamus.
TS20: choroid plexus	Absent		8I