Probe:	Fzd8 cDNA6
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Strong	Regionally restricted	1H	Expression is ventral.
TS15: telencephalon	Strong	Regionally restricted	1H	Expression is ventral.
TS15: future hindbrain	Absent		1H
TS15: future midbrain	Absent		1H

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Weak	Regionally restricted	2H	Expression is anterior ventral.
TS17: telencephalon	Weak	Regionally restricted	2H	Expression is ventral.
TS17: hindbrain	Absent		2H
TS17: midbrain	Absent		2H

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Strong	Regionally restricted	3H	Expression is in the optic recess and preoptic area and is weaker in the postoptic area surrounding the mammillary recess.
TS20: ganglionic eminence	Present		3H
TS20: midbrain roof plate	Present	Regionally restricted	3H	Expression is in the tectum.
TS20: midbrain-hindbrain junction	Present	Regionally restricted	3H	Expression is in a small domain at the dorsal midbrain/hindbrain boundary.

Structure	Level	Pattern	Image	Note
TS20: midbrain roof plate	Present	Regionally restricted	6G	Expression is along the anterior/posterior axis in the rostral tetcum.
TS20: midbrain-hindbrain junction	Present	Regionally restricted	6G	Expression is dorsal.
TS20: hindbrain	Weak		6G
TS20: midbrain lateral wall	Weak		6G
TS20: midbrain	Weak	Regionally restricted	6G	There is a gap in expression in the caudal cephalic flexure.

Structure	Level	Pattern	Image	Note
TS20: midbrain lateral wall	Weak	Regionally restricted	6G'	Expression is lower surrounding the floor plate. Expression is absent or only weakly in the basal plate and in the most ventral part of the alar plate.
TS20: midbrain roof plate	Weak		6G'	Expression is absent or only weakly expressed.
TS20: midbrain floor plate	Absent		6G'

Structure	Level	Pattern	Image	Note
TS17: midbrain	Absent		7E

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	8H	Expression is strongest around the pallial/subpallial boundary (the cortical antihem) and in the lateral pallium. There is a decreasing gradient of expression in the dorsal pallium. Expression is absent in the medial pallium.
TS20: lateral ganglionic eminence	Strong		8H
TS20: medial ganglionic eminence	Present	Graded	8H	There are decreasing gradients of expression.
TS20: preoptic area	Present	Graded	8H	There are decreasing gradients of expression.
TS20: telencephalon subventricular zone	Present	Regionally restricted	8H	Expression is in the subpallium.
TS20: telencephalon ventricular layer	Present	Regionally restricted	8H	Expression is in the subpallium.