Probe:	Fzd7 cDNA5
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Strong	Regionally restricted	1G	Expression is ventral with a sharp border approximating the position of the midbrain/hindbrain boundary.
TS15: future midbrain	Present	Regionally restricted	1G	Expression is in the rostro-ventral midbrain with a gap at the caudal cephalic flexure.
TS15: diencephalon	Weak	Non-Uniform	1G	Expression is weak in a small domain of the ventral diencephalon and at the dorsal telencephalic/diencephalic boundary.
TS15: telencephalon	Weak	Regionally restricted	1G	Expression is in the dorsal telencephalon and at the dorsal telencephalic/diencephalic boundary.
TS15: head mesenchyme	Weak	Regionally restricted	1G	Expression is in the mesenchyme underlying the rostral telencephalon.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Strong	Regionally restricted	2G	Expression is ventral with a sharp border approximating the position of the midbrain/hindbrain boundary.
TS17: head mesenchyme	Present	Regionally restricted	2G	Expression is in the rostral head mesenchyme.
TS17: diencephalon	Weak	Regionally restricted	2G	Expression is in a small domain in the ventral diencephalon and in the ventral rostral diencephalon abutting the optic recess caudally.
TS17: telencephalon	Weak	Regionally restricted	2G	Expression is in the dorsal telencephalon up to the telencephalic/diencephalic boundary.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Strong		3G
TS20: hindbrain	Strong	Regionally restricted	3G	Expression is ventral up to the midbrain/hindbrain boundary with a gap within the most caudal part of the cephalic flexure.
TS20: midbrain	Strong	Regionally restricted	3G	Expression is in the cephalic flexure (ventral mesencephalon).
TS20: diencephalon	Present	Regionally restricted	3G	Expression is in the cephalic flexure (ventral mesencephalon and diencephalon), in the subthalamic and postoptic area, around the mammillary recess, and in the optic recess.
TS20: preoptic area	Present		3G
TS20: telencephalon	Present	Regionally restricted	3G	Expression is in the caudal part of the dorso-medial telencephalon (including the hippocampal anlage).
TS20: thalamus	Weak	Regionally restricted	3G	Expression is in the dorsal thalamus.
TS20: ganglionic eminence	Weak	Regionally restricted	3G	Expression is in a caudorostral gradient.
TS20: midbrain roof plate	Trace		3G
TS20: cerebral cortex	Trace	Graded	3G	Expression is in a rostro-caudal gradient with highest expression in the frontal cortex and septal area.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Strong	Regionally restricted	4F	Expression is in the ventral anterior hindbrain (r1) and weaker expression in the dorsal anterior hindbrain (rhombic lip) that end at the level of the midbrain/hindbrain boundary.

Structure	Level	Pattern	Image	Note
TS17: midbrain ventricular layer	Weak	Regionally restricted	4F'	Expression is the alar/basal boundary.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	5G	There is a gap in expression in the caudal cephalic flexure.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Regionally restricted	6F	Expression is strongest in the ventral anterior hindbrain (ventral r1) with a sharp rostral border at the midbrain/hindbrain boundary.
TS20: midbrain	Present	Regionally restricted	6F	There is a gap in expression at the caudal cephalic flexure, corresponding approximately to the ventral position of the midbrain/hindbrain boundary (MHB) and is weak in the remaining cephalic flexure. A similar domain of very weak to absent expression is dorsally in a region corresponding to the caudal tectum (presumptive inferior colliculus) just rostral to the MHB. Lower levels are in the remaining dorsal midbrain.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Strong	Regionally restricted	6F'	Expression is in the ventricular zone of the ventral and lateral midbrain, including the basal plate and ventral alar plate, but is absent from the ventral midline (floor plate).
TS20: midbrain ventricular layer	Strong	Regionally restricted	6F'	Expression is in the ventricular zone of the ventral and lateral midbrain, including the basal plate and ventral alar plate, but is absent from the ventral midline (floor plate).
TS20: midbrain roof plate	Trace		6F'
TS20: midbrain floor plate	Absent		6F'

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Graded	8G	Expression is high dorsally to low ventrally. Expression is in the lateral pallium and in the dorsomedial telencephalon including the hippocampus anlage.
TS20: lateral ganglionic eminence	Strong	Regionally restricted	8G	Expression is in the ventricular/subventricular zone.
TS20: choroid plexus	Present		8G
TS20: cortical hem	Present		8G
TS20: diencephalon	Present	Regionally restricted	8G	Expression is in the thalamic eminence.
TS20: medial ganglionic eminence	Weak	Regionally restricted	8G	Expression is mostly in the ventricular/subventricular zone.
TS20: preoptic area	Weak	Regionally restricted	8G	Expression is mostly in the ventricular/subventricular zone.