Probe:	Fzd6 cDNA6
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Strong	Regionally restricted	1F	In the ventral neural tube, expression is strongest in the caudal mesencephalon immediately adjacent to the rostral border of the isthmus
TS15: future hindbrain	Strong	Regionally restricted	1F	In the dorsal neural tub expression is restricted mostly to the anterior hindbrain just caudal to the isthmus in a region corresponding to the rhombic lip.
TS15: midbrain-hindbrain junction	Present		1F
TS15: future brain	Present	Spotted	1F
TS15: head mesenchyme	Present	Spotted	1F
TS15: telencephalon	Weak	Regionally restricted	1F	Expression is rostral.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Strong	Regionally restricted	2F	Expression is in the caudal cephalic flexure and dorso-caudal midbrain abutting the midbrain/hindbrain boundary.
TS17: telencephalon	Strong	Regionally restricted	2F	Expression is in the caudo-medial dorsal telencephalon.
TS17: midbrain-hindbrain junction	Present	Regionally restricted	2F	Expression is at the rostral border.
TS17: head mesenchyme	Weak	Spotted	2F
TS17: brain	Weak	Spotted	2F	Expression is throughout the anterior neural tube.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	3F	Expression is in the thalamic eminence just below the nascent choroid plexus in the diencephalonand in the caudal cephalic flexure.
TS20: midbrain	Present		3F	Expression is dorsal just rostral to the midbrain/hindbrain boundary.
TS20: brain	Present	Regionally restricted	3F	Expression is in a spotted pattern throughout the anterior neural tube.
TS20: head mesenchyme	Present	Spotted	3F

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	4E	Expression is in the caudal cephalic flexure (ventral midbrain) and in the dorsal midbrain.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Strong	Regionally restricted	4E'	Expression is in two stripes of strong expression within the basal plate and abutting the medial floor plate.
TS17: midbrain floor plate	Weak	Regionally restricted	4E'	Expression is in the medial floor plate extending from the ventricular zone into the marginal zone.
TS17: midbrain marginal layer	Weak	Regionally restricted	4E'	Expression is in the medial floor plate extending from the ventricular zone into the marginal zone.
TS17: midbrain ventricular layer	Weak	Regionally restricted	4E'	Expression is in the medial floor plate extending from the ventricular zone into the marginal zone.
TS17: midbrain roof plate	Absent		4E'

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present		5F	Expression is in the caudal cephalic flexure and dorso-caudal midbrain abutting the midbrain/hindbrain boundary.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Strong	Regionally restricted	6E	Expression is in the caudal cephalic flexure and the dorsal midbrain just rostral to the midbrain/hindbrain boundary. Expression is weak and spotted in the remaining regions.
TS20: hindbrain	Weak	Spotted	6E

Structure	Level	Pattern	Image	Note
TS20: midbrain	Strong	Regionally restricted	6E'	Expression is in the ventricular zone (VZ) of the basal plate, sparing the ventral midline (floor plate). Weak mFzd6 expression is visible in the VZ and Sub-VZ of the mesencephalic roof and dorsal alar plate. Furthermore expression is spotted.
TS20: midbrain floor plate	Strong	Regionally restricted	6E'	Expression is in the ventricular zone (VZ) of the basal plate, sparing the ventral midline (floor plate). Weak mFzd6 expression is visible in the VZ and Sub-VZ of the mesencephalic roof and dorsal alar plate. Furthermore expression is spotted.
TS20: midbrain ventricular layer	Strong	Regionally restricted	6E'	Expression is in the ventricular zone (VZ) of the basal plate, sparing the ventral midline (floor plate). Weak mFzd6 expression is visible in the VZ and Sub-VZ of the mesencephalic roof and dorsal alar plate. Furthermore expression is spotted.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	8F	Expression is in a small domain of the thalamic eminence, just below the nascent choroid plexus.
TS20: telencephalon	Present	Spotted	8F	Expression is especially in the subpallium.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	9A' , 9A	Expression is in the neuroepithelium.
TS20: brain vascular element	Present		9A' , 9A
TS20: head mesenchyme	Present		9A' , 9A

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	9B' , 9B	Expression is in the neuroepithelium.
TS20: brain vascular element	Present		9B' , 9B
TS20: head mesenchyme	Present		9B' , 9B