Probe:	Fzd2 cDNA4
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Uniform	1B
TS15: future hindbrain	Present	Uniform	1B
TS15: future midbrain	Present	Uniform	1B
TS15: telencephalon	Present	Uniform	1B
TS15: head mesenchyme	Present		1B

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Strong	Regionally restricted	2B	Expression is in the rostral head mesenchyme.
TS17: telencephalon	Weak	Regionally restricted	2B	Expression is ventral.
TS17: hindbrain	Present	Regionally restricted	2B	Expression is ventral and is is very low in the dorsal part of the anterior rhombencephalon.
TS17: diencephalon	Present	Regionally restricted	2B	Expression is mostly ventral and is very low in the dorsal part of the anterior diencephalon. There is a dorsal boundary and ventral gap in expression corresponding to the position of the zona limitans intrathalamica.
TS17: midbrain	Present	Regionally restricted	2B	Expression is mostly ventral and is very low in the dorsal part of the anterior midbrain. There is a gap in expression in the caudal cephalic flexure/ventro-caudal mesencephalon, extending just rostral to the isthmus.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Strong		3B
TS20: midbrain	Present	Regionally restricted	3B	There is an expression gap in the the caudal cephalic flexure approximating the position of the midbrain/hindbrain boundary.
TS20: cerebellum primordium	Present		3B
TS20: cerebral cortex	Present		3B
TS20: diencephalon	Present		3B
TS20: ganglionic eminence	Present		3B
TS20: hindbrain	Present		3B
TS20: mammillary area	Present		3B
TS20: pons	Present		3B
TS20: preoptic area	Present		3B
TS20: prethalamus	Present		3B
TS20: telencephalon	Present		3B
TS20: thalamus	Present		3B
TS20: midbrain roof plate	Present	Regionally restricted	3B	Expression is in the tectum and is absent in the pretectum and the most caudal part of the tectum.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	4C	Expression is in the ventral part of the anterior hindbrain (rhombomere 1, r1).
TS17: midbrain	Present	Regionally restricted	4C	Expression is ventral and weak expression in the dorsal midbrain. There is a gap in expression along the anterior/posterior axis of the caudal ventral midbrain corresponding to the caudal cephalic flexure.
TS17: head mesenchyme	Present	Regionally restricted	4C	Expression is in the mesenchyme overlying the areas of expression.
TS17: midbrain-hindbrain junction	Absent		4C

Structure	Level	Pattern	Image	Note
TS17: midbrain floor plate	Present		4C'
TS17: midbrain roof plate	Present		4C'
TS17: midbrain ventricular layer	Weak	Regionally restricted	4C'	Expression is throughout the dorsal/ventral extent.
TS17: midbrain lateral wall	Weak	Regionally restricted	4C'	Expression is throughout the dorsal/ventral extent of the subventricular zone.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	5D	There is a gap in expression in the caudal cephalic flexure.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Regionally restricted	6C	Expression is in the ventral midline of the rostral hindbrain (r1).
TS20: midbrain	Strong	Regionally restricted	6C	Expression is in the ventral midline of the caudal midbrain with lower expression along the anterior/posterior axis of the caudal cephalic flexure.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Strong	Regionally restricted	6C'	Expression is dorsal in the ventricular zone and subventricular zone (VZ/SVZ) and at lower levels in the marginal zone of the alar plate with sharp ventral border at the level of the alar/basal boundary. Ventral expression is mostly in the VZ/SVZ of the mesencephalic basal and floor plate.
TS20: midbrain floor plate	Strong	Regionally restricted	6C'
TS20: midbrain marginal layer	Strong	Regionally restricted	6C'
TS20: midbrain ventricular layer	Strong	Regionally restricted	6C'

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	8B	Expression is in the pallial/subpallial boundary and is weak in the dorsal and medial pallium.
TS20: lateral ganglionic eminence	Present	Regionally restricted	8B	Expression is in the ventricular, subventricular zone, and marginal zone.
TS20: medial ganglionic eminence	Present	Regionally restricted	8B	Expression is in the ventricular, subventricular zone, and marginal zone.
TS20: cortical hem	Weak		8B
TS20: choroid plexus	Absent		8B