Antibody:	anti-LIMK2
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Cell Type	Level	Pattern	Image	Note
TS22: central nervous system	neuron	Present	Regionally restricted	6A , 7D	Expression in postmitotic neurons.
TS22: main olfactory bulb		Strong		6A
TS22: spinal cord		Strong		6A
TS22: cerebellum		Present		6A
TS22: hindbrain		Present		6A
TS22: hypothalamus		Present		6A
TS22: midbrain		Present		6A

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus	neuron	Strong		6C

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebral cortex	neuron	Strong		6E

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum	Purkinje cell	Strong		6F

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum	Purkinje cell	Strong		6G

Structure	Level	Pattern	Image	Note
TS28: brain ependyma	Strong		6I
TS28: choroid plexus	Strong		6I

Structure	Cell Type	Level	Pattern	Image	Note
TS28: photoreceptor layer		Present		6J	Expression in all layers of retina including photoreceptors, outer nuclear layer, inner nuclear layer; inner plexiform layer and ganglion cell layer.
TS28: retina inner plexiform layer		Present		6J	Expression in all layers of retina including photoreceptors, outer nuclear layer, inner nuclear layer; inner plexiform layer and ganglion cell layer.
TS28: retina outer nuclear layer		Present		6J	Expression in all layers of retina including photoreceptors, outer nuclear layer, inner nuclear layer; inner plexiform layer and ganglion cell layer.
TS28: retina inner nuclear layer	amacrine cell	Strong		6J	Highest expression in horizontal bipolar cells and amacrine cells of inner nuclear layer.
TS28: retina inner nuclear layer	retinal bipolar neuron	Strong		6J	Highest expression in horizontal bipolar cells and amacrine cells of inner nuclear layer.
TS28: retina ganglion cell layer	retinal ganglion cell	Strong		6J	Highest expression in ganglion cells of ganglion cell layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lung		Very strong	Widespread	7A	Very high expression in lung in all cells.
TS28: alveolus epithelium		Very strong	Regionally restricted	7A	Very high expression in epithelial cells lining lumen of respiratory bronchioles and alveoli.
TS28: respiratory bronchiole epithelium		Very strong	Regionally restricted	7A	Very high expression in epithelial cells lining lumen of respiratory bronchioles and alveoli.
TS28: lung epithelium	club cell	Present		7A	Expression in non-ciliated Clara cells (club cells) and terminally differentiated ciliated cells.
TS28: lung epithelium	lung multiciliated epithelial cell	Present		7A	Expression in non-ciliated Clara cells (club cells) and terminally differentiated ciliated cells.
TS28: lung vascular element		Present	Regionally restricted	7A	Expression in epithelial cells lining arterioles.

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Strong		7B

Structure	Cell Type	Level	Pattern	Image	Note
TS28: crypt of lieberkuhn		Strong		7C
TS28: intestine smooth muscle		Strong		7C
TS28: intestine	intestine goblet cell	Strong		7C

Structure	Cell Type	Level	Pattern	Image	Note
TS28: stomach	parietal cell	Strong		7D	High levels of expression in chief cells (peptic cells) and parietal cells of stomach.
TS28: stomach	peptic cell	Strong		7D	High levels of expression in chief cells (peptic cells) and parietal cells of stomach.
TS28: stomach	mucous neck cell of gastric gland	Moderate		7D	Moderate levels of expression in mucous neck cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: liver		Present	Widespread	7E	Expression in all cells.
TS28: liver parenchyma		Present		7E
TS28: liver sinusoid	endothelial cell of hepatic sinusoid	Present		7E

Structure	Cell Type	Level	Pattern	Image	Note
TS28: thymus		Strong	Regionally restricted	7F
TS28: thymus cortex	thymocyte	Strong		7F	Strong expression in cortical thymocytes (more immature T-cells).
TS28: thymus medullary core	mature T cell	Strong		7F	Strong expression in more mature T-cells in medulla.
TS28: thymus medulla epithelium	medullary thymic epithelial cell	Present		7F	Expression in epithelial reticular cells.

Structure	Level	Pattern	Image	Note
TS28: spleen red pulp	Strong		7G
TS28: spleen white pulp	Strong		7G

Structure	Level	Pattern	Image	Note
TS28: dermis	Strong		7H
TS28: epidermis	Strong		7H
TS28: hair bulb	Strong		7H
TS28: hair follicle	Strong		7H
TS28: sebaceous gland	Strong		7H

Structure	Level	Pattern	Image	Note
TS28: metanephros	Moderate	Widespread	7I	Moderate expression in most cells.
TS28: distal convoluted tubule	Present		7I	Expression in all tubular structures including proximal and distal convoluted tubules.
TS28: renal proximal convoluted tubule	Present		7I	Expression in all tubular structures including proximal and distal convoluted tubules.
TS28: renal vasculature	Present	Regionally restricted	7I	Expression in endothelial cells lining arterioles.
TS28: glomerular tuft	Absent		7I

Structure	Level	Pattern	Image	Note
TS28: endometrium glandular epithelium	Very strong		8A	Very high levels of expression in endometrial cells lining uterine glands.
TS28: endometrium luminal epithelium	Very strong		8A	Very high levels of expression in endometrial cells lining lumen of uterus.
TS28: myometrium	Trace		8A	Very low levels of expression in myometrium.

Structure	Level	Pattern	Image	Note
TS28: corpus luteum	Strong		8B
TS28: ovary follicle	Strong		8B
TS28: surface epithelium of ovary	Moderate		8B

Structure	Level	Pattern	Image	Note
TS28: spermatid	Very strong	Regionally restricted	8E	Very high expression exclusively in elongated spermatids.

Structure	Level	Pattern	Image	Note
TS28: spermatid	Very strong	Regionally restricted	8F	Very high expression exclusively in elongated spermatids.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: brain	ependymal cell	Strong
TS28: brain	neuron	Strong
TS28: brain	Purkinje cell	Strong
TS28: brain	glial cell	Absent
TS28: thymus	thymocyte	Present	Regionally restricted		Expression in cortical and medullary thymocytes.
TS28: spleen	follicular B cell	Present
TS28: stomach	parietal cell	Strong
TS28: stomach	peptic cell	Strong
TS28: stomach	mucous neck cell of gastric gland	Present
TS28: crypt of lieberkuhn		Strong
TS28: intestine	enteric smooth muscle cell	Strong
TS28: intestine	intestine goblet cell	Strong
TS28: bronchiole	lung multiciliated epithelial cell	Strong
TS28: dermis		Present
TS28: epidermis		Present
TS28: hair follicle		Strong
TS28: hair bulb		Present
TS28: sebaceous gland		Present
TS28: distal convoluted tubule		Present
TS28: renal proximal convoluted tubule		Present
TS28: collecting duct		Present
TS28: renal vasculature		Present
TS28: glomerular tuft		Absent
TS28: heart	cardiac muscle cell	Present
TS28: spermatid		Strong	Regionally restricted		High expression in elongated spermatids. No expression in early spermatids.
TS28: photoreceptor layer		Present
TS28: retina outer nuclear layer		Present
TS28: retina inner nuclear layer		Strong
TS28: retina outer plexiform layer		Strong
TS28: retina ganglion cell layer		Strong
TS28: retina inner plexiform layer		Strong

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatocyte		Present
TS28: Sertoli cell	Sertoli cell	Present

Structure	Level	Pattern	Image	Note
TS28: endometrium	Strong	Regionally restricted		Expression in tubular glands
TS28: myometrium	Weak
TS28: corpus luteum	Strong
TS28: ovary follicle	Strong
TS28: surface epithelium of ovary	Present