Gene Expression Data
Assay Details
Assay
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Reference:
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J:311233
Huang YT, et al., Lateral cortical Cdca7 expression levels are regulated by Pax6 and influence the production of intermediate progenitors. BMC Neurosci. 2017 Jun 5;18(1):47
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7286370
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Gene symbol:
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Cdca7
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Gene name:
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cell division cycle associated 7
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Probe:
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Cdca7 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1E/E':
embryonic day 12.5
(more )
Specimen
1E/E':
(close )
Genetic Background:
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involves: CD-1
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Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
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Specimen
1F/F':
embryonic day 12.5
(more )
Specimen
1F/F':
(close )
Genetic Background:
|
involves: CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
|
1F
,
1F'
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Expression pattern was opposite to that of Pax6, with lower levels on the cortical side of the pallial-subpallial boundary, in the lateral cortex.
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Specimen
1G/G':
(close )
Genetic Background:
|
involves: CD-1
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Mutant Allele(s):
|
Pax6Sey/Pax6Sey
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Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1H/H':
(close )
Genetic Background:
|
involves: CD-1
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
|
1H
,
1H'
|
Expression was abnormal in the cortex and showed no obvious decrease on the cortical side of the pallial-subpallial boundary.
|
Specimen
2C/C':
embryonic day 14.5
(more )
Specimen
2C/C':
(close )
Genetic Background:
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involves: CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
2D/D':
(close )
Genetic Background:
|
involves: CD-1
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Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: cerebral cortex |
Strong |
Regionally restricted |
2D
,
2D'
|
Expression was abnormal in the cortex and showed no obvious decrease on the pallial side of the pallial-subpallial boundary. Expression in subpallium continued across the boundary deep into the lateral cortex where they now overlapped high Pax6 expression.
|
TS22: lateral ganglionic eminence |
Strong |
|
2D'
,
2D
|
|
Specimen
not shown:
embryonic day 13.5
(more )
Note: Quantitative analysis was summarized in Fig 3E and 3F.
Specimen
not shown:
(close )
Genetic Background:
|
involves: CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Quantitative analysis was summarized in Fig 3E and 3F.
|
Specimen
not shown:
embryonic day 13.5; Pax6Sey/ Pax6Sey
(more )
Note: Quantitative analysis was summarized in Fig 3E and 3F.
Specimen
not shown:
(close )
Genetic Background:
|
involves: CD-1
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Quantitative analysis was summarized in Fig 3E and 3F.
|
|