Gene Expression Data
Assay Details
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Assay
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Reference:
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J:257338
Dougherty GW, et al., DNAH11 Localization in the Proximal Region of Respiratory Cilia Defines Distinct Outer Dynein Arm Complexes. Am J Respir Cell Mol Biol. 2016 Aug;55(2):213-24
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7286226
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Gene symbol:
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Dnah11
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Gene name:
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dynein, axonemal, heavy chain 11
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Reporter:
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GFP
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Antibody:
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anti-GFP
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Detection system:
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Secondary antibody
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Results
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Specimen
2D:
postnatal; Dnah11tm1.1Bknr/Dnah11 +
(more  )
Note: Double labeled: green - Dnah11; red - acetylated alpha-tubulin.
Specimen
2D:
(close  )
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Genetic Background:
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involves: 129S1/Sv * FVB/N
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Mutant Allele(s):
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Dnah11tm1.1Bknr/Dnah11+
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Dnah11; red - acetylated alpha-tubulin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: respiratory system |
respiratory epithelial cell |
Present |
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2D GFP-DNAH11
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2D merge
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Expression was localized to the proximal region of ciliary axonemes of respiratory cells. The ciliary axonemes were counterstained with acetylated alpha-tubulin.
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Specimen
2E:
embryonic day 7.75; Dnah11tm1.1Bknr/Dnah11 +
(more )
Note: Double labeled: green - Dnah11; red - acetylated alpha-tubulin.
Specimen
2E:
(close )
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Genetic Background:
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involves: 129S1/Sv * FVB/N
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Mutant Allele(s):
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Dnah11tm1.1Bknr/Dnah11+
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Age:
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embryonic day 7.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Dnah11; red - acetylated alpha-tubulin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS12: node |
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Present |
Regionally restricted |
2EI
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2E
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Expression was localized throughout the entire axoneme in motile monocilia of the left-right organizer. Expression was not detectable in nonmotile cilia of the node.
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| TS12: embryo endoderm |
endodermal cell |
Absent |
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2E
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2EII
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Expression was not detectable in primary cilia of endodermal cells.
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Analysis Tools