Tg(Rr194,Myh2-YFP,Myh1-tdTomato,Myh4-CFP)EnhMair
Transgene Detail
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| Symbol: |
Tg(Rr194,Myh2-YFP,Myh1-tdTomato,Myh4-CFP)EnhMair |
| Name: |
transgene insertion Enh, Pascal Maire |
| MGI ID: |
MGI:7285976 |
| Synonyms: |
fMyh Enh+, Myh1Tomato, Myh2YFP, Myh4CFP |
| Transgene: |
Tg(Rr194,Myh2-YFP,Myh1-tdTomato,Myh4-CFP)EnhMair Location: unknown
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| Alliance: |
Tg(Rr194,Myh2-YFP,Myh1-tdTomato,Myh4-CFP)EnhMair page
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| Transgene Type: |
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Transgenic (Reporter) |
| Mutation: |
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Insertion
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Mutation details: The transgene insert comprises a modified BAC RP23-61C14 (containing the 3' end of Myh3, Rr194, Myh2, Myh1, Myh4 and the 5' end of Myh8) where the following modifications were made: a vector containing the YFP reporter gene, a poly(A) signal cassette and a loxP site flanked kanamycin resistance gene cassette was inserted at the ATG start site of the Myh2 gene; a vector containing the tdTomato reporter gene, a poly(A) signal cassette and a lox2272 site flanked kanamycin resistance gene cassette was inserted at the ATG start site of the Myh1 gene; a vector containing the CFP reporter gene, a poly(A) signal cassette and a loxN site flanked kanamycin resistance gene cassette was inserted at the ATG start site of the Myh4 gene. The kana cassettes were removed through subsequent cre-mediated recombination. Super enhancer Rr194 controls expression of the Myh genes. Two copies of this transgene are integrated into the genome.
(J:322002)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:322002 Dos Santos M, et al., A fast Myosin super enhancer dictates muscle fiber phenotype through competitive interactions with Myosin genes. Nat Commun. 2022 Feb 24;13(1):1039 |
| All: |
1 reference(s) |
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Analysis Tools
