Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Cpne4em1(flpo*)Ngai
Name:	copine IV; endonuclease-mediated mutation 1, John Ngai
MGI ID:	MGI:7286056
Gene:	Cpne4  Location: Chr9:104443900-104911747 bp, + strand  Genetic Position: Chr9, 56.61 cM, cytoband F1
Alliance:	Cpne4em1(flpo*)Ngai page

Mouse Generated:	Earliest citation of chimera generation: J:101977
Parent Cell Line:	v6.5 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Allele Type:    Endonuclease-mediated (Recombinase) Mutation:    Insertion   Cpne4em1(flpo*)Ngai expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Cpne4 (MGI:1921270)   Mutation details: Using CRISPR/Cas9 technology, a P2A-Flpo (encoding a viral 2A oligopeptide that mediates ribosomal skipping and a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice) was inserted into the targetted STOP codon of the Copine 4 gene (Cpne4) on chromosome 9 in embryonic stem cells. This flpo coding sequence was further codon modified; standard flpo PCR primers may not anneal. Chimeric mice were generated by aggregating ES cells with morula-stage embryos and bred for germline transmission. (J:101977)

Activity:	Tissue activity of this recombinase allele
Driver:	Cpne4 (mouse) Summary of all recombinase alleles driven by Cpne4. Alliance mouse Cpne4 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cpne4 Mutation:	24 strains or lines available

Original:	J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:	1 reference(s)