Cplx3em1(flpo*)Ngai
Endonuclease-mediated Allele Detail
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Symbol: |
Cplx3em1(flpo*)Ngai |
Name: |
complexin 3; endonuclease-mediated mutation 1, John Ngai |
MGI ID: |
MGI:7286055 |
Gene: |
Cplx3 Location: Chr9:57507275-57513564 bp, - strand Genetic Position: Chr9, 31.11 cM
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Alliance: |
Cplx3em1(flpo*)Ngai page
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Allele Type: |
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Endonuclease-mediated (Recombinase) |
Mutation: |
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Insertion
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Cplx3em1(flpo*)Ngai expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: Using CRISPR/Cas9 technology, a P2A-Flpo (encoding a viral 2A oligopeptide that mediates ribosomal skipping and a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice) was inserted into the targetted STOP codon of the Complexin-3 gene (Cplx3) on chromosome 9 in embryonic stem cells. This flpo coding sequence was further codon modified; standard flpo PCR primers may not anneal. Chimeric mice were generated by aggregating ES cells with morula-stage embryos and bred for germline transmission.The ampicillin cassette is still present in this allele.
(J:101977)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Cplx3
(mouse)
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Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
All: |
1 reference(s) |
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