Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Hpseem1(cre)Ngai
Name:	heparanase; endonuclease-mediated mutation 1, John Ngai
MGI ID:	MGI:7286051
Gene:	Hpse  Location: Chr5:100827350-100867582 bp, - strand  Genetic Position: Chr5, 48.51 cM
Alliance:	Hpseem1(cre)Ngai page

Germline Transmission:	Earliest citation of germline transmission: J:101977
Parent Cell Line:	v6.5 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Allele Type:    Endonuclease-mediated (Epitope tag, Recombinase) Mutation:    Insertion   Hpseem1(cre)Ngai expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Hpse (MGI:1343124)   Mutation details: Using CRISPR/Cas9 technology, a P2A-Cre (encoding a viral 2A oligopeptide that mediates ribosomal skipping and a codon-optimized cre gene) and a myc-tag were inserted into the targetted STOP codon of the Heparanase gene (Hpse) on chromosome 9 in embryonic stem cells. Chimeric mice were generated by aggregating ES cells with morula-stage embryos and bred for germline transmission. (J:101977)

Activity:	Tissue activity of this recombinase allele
Driver:	Hpse (mouse) Summary of all recombinase alleles driven by Hpse. Alliance mouse Hpse gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hpse Mutation:	37 strains or lines available

Original:	J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:	1 reference(s)