Gene Expression Data
Assay Details
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Assay
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Reference:
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J:136642
Matera I, et al., A sensitized mutagenesis screen identifies Gli3 as a modifier of Sox10 neurocristopathy. Hum Mol Genet. 2008 Jul 15;17(14):2118-31
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7286028
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Gene symbol:
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Pmel
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Gene name:
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premelanosome protein
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Probe:
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Si probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6E:
embryonic day 11.5
(more  )
Specimen
6E:
(close  )
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Genetic Background:
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involves: BALB/cJ * C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6F:
embryonic day 11.5
(more )
Specimen
6F:
(close )
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Genetic Background:
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involves: BALB/cJ * C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: trunk |
melanoblast |
Present |
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6F
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Specimen
6G:
(close )
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Genetic Background:
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involves: BALB/cJ * C57BL/6J
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Mutant Allele(s):
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Gli3Mos1/Gli3Mos1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: craniocervical region |
melanoblast |
Present |
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6G
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Expression in head, cervical and tail regions similar to wildtype embryos.
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| TS19: tail |
melanoblast |
Present |
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6G
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Expression in head, cervical and tail regions similar to wildtype embryos.
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Specimen
6H:
(close )
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Genetic Background:
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involves: BALB/cJ * C57BL/6J
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Mutant Allele(s):
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Gli3Mos1/Gli3Mos1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: trunk |
melanoblast |
Weak |
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6H
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Expression in very few melanoblasts in trunk region between forelimb and hindlimb compared to wildtype regions. No ectopic expression.
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Specimen
8A:
embryonic day 11.5
(more )
Specimen
8A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: embryo |
melanoblast |
Present |
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8A
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Specimen
8B:
embryonic day 11.5
(more )
Specimen
8B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: trunk |
melanoblast |
Present |
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8B
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Specimen
8C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Gli3tm1Urt/Gli3tm1Urt
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: embryo |
melanoblast |
Present |
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8C
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Expression patterns similar to wild type embryos.
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Specimen
8D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Gli3tm1Urt/Gli3tm1Urt
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: trunk |
melanoblast |
Present |
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8D
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Expression patterns similar to wild type embryos.
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Analysis Tools