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Gene Expression Data
Assay Details
Assay
Reference: J:309381 Sadegh C, et al., Synthetic modified Fezf2 mRNA (modRNA) with concurrent small molecule SIRT1 inhibition enhances refinement of cortical subcerebral/corticospinal neuron identity from mouse embryonic stem cells. PLoS One. 2021;16(9):e0254113
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7284345
Gene symbol: Sirt1
Gene name: sirtuin 1
Antibody: anti-Sirt1
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 4A left: postnatal day 4 (more )
Note: Triple labeled: green - Sirt1; cyan - Bcl11b (assay MGI:7284347); red - Satb2 (assay MGI:7284346).
Structure Level Pattern Image Note
TS28: cortical layer II Present 4A left
TS28: cortical layer III Present 4A left
TS28: cortical layer VI Present 4A left
TS28: cortical subplate Present 4A left
Specimen 4A middle: postnatal day 4 (more )
Note: Triple labeled: green - Sirt1; cyan - Bcl11b (assay MGI:7284347); red - Satb2 (assay MGI:7284346).
Structure Level Pattern Image Note
TS28: cortical layer II Present 4A middle
TS28: cortical layer III Present 4A middle
TS28: cortical layer VI Present 4A middle
TS28: cortical subplate Present 4A middle
Specimen 4A right: postnatal day 4 (more )
Note: Triple labeled: green - Sirt1; cyan - Bcl11b (assay MGI:7284347); red - Satb2 (assay MGI:7284346).
Structure Level Pattern Image Note
TS28: cortical layer II Present 4A right
TS28: cortical layer III Present 4A right
TS28: cortical layer VI Present 4A right
TS28: cortical subplate Present 4A right
Specimen 4B/B': embryonic day 18.5 (more )
Note: Triple labeled: green - Sirt1; cyan - Bcl11b (assay MGI:7284347); red - Satb2 (assay MGI:7284346).
Structure Level Pattern Image Note
TS26: neocortex Present 4B left , 4B merge , 4B' middle Near complete co-localization with Satb2+ callosal projection neurons in layers II/III, V, and VI, and exclusion by Bcl11b+ subcerebral projection neurons/corticospinal neurons in layer V.
Specimen 4C: postnatal day 4 (more )
Note: Triple labeled: green - Sirt1; cyan - Bcl11b (assay MGI:7284347); red - Satb2 (assay MGI:7284346).
Structure Level Pattern Image Note
TS28: neocortex Present 4C left , 4C merge Expression in deep layers of motor cortex was almost completely restricted to Satb2+ neurons, excluded from increasingly mature Bcl11b+ subcerebral projection neurons/corticospinal neurons.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory