Probe:	Tie-2 cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Probe was subjected to limited alkaline hydrolysis to about 400-bp.

Structure	Level	Pattern	Image	Note
TS26: renal small blood vessel	Present		3E
TS26: glomerular tuft	Absent		3E

Structure	Level	Pattern	Image	Note
TS26: renal small blood vessel	Present	Regionally restricted	4G	Expression is in capillaries aligned in parallel to tubules.

Structure	Level	Pattern	Image	Note
TS27: renal cortical capillary	Present	Regionally restricted	5D	Expression is in capillaries in the interstitium around proximal tubules.
TS27: blood vessel endothelium	Present	Regionally restricted	5D	Expression is in the endothelial lining of cortical arteries.

Structure	Level	Pattern	Image	Note
TS27: renal medullary capillary	Present	Regionally restricted	6G	Expression is in capillaries running parallel to the tubules and in inner medullary capillaries.

Structure	Level	Pattern	Image	Note
TS27: renal medullary capillary	Present	Regionally restricted	6H	Expression is in capillaries running parallel to the tubules and in inner medullary capillaries.

Structure	Level	Pattern	Image	Note
TS27: glomerular tuft	Present		7C

Structure	Level	Pattern	Image	Note
TS27: endothelium of renal artery	Present		7F
TS27: renal cortical arterial system	Present		7F

Structure	Level	Pattern	Image	Note
TS27: renal medullary capillary	Present	Regionally restricted	8G	Expression is in cells in close association with capillaries of the vasa recta and is sparse or absent in the interbundle region.

Structure	Level	Pattern	Image	Note
TS27: renal medullary capillary	Present	Regionally restricted	8H	Expression is in cells in close association with capillaries of the vasa recta and is sparse or absent in the interbundle region.