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Gene Expression Data
Assay Details
Assay
Reference: J:58604 Debrand E, et al., Functional analysis of the DXPas34 locus, a 3' regulator of Xist expression. Mol Cell Biol. 1999 Dec;19(12):8513-25
Assay type: RNA in situ
MGI Accession ID: MGI:7281486
Gene symbol: DXPas34
Gene name: DNA segment, Chr X, Pasteur Institute 34
Probe: DXPas34 probe
Probe preparation: Antisense labelled with Texas Red RNA
Results
Specimen 2M: embryonic day 3.5 (more )
Note: Early morulas: 8-16 cells. Double labeled: red - DXPas34; green - Xist (assay MGI:7281465).
Structure Level Pattern Image Note
TS4: compacted morula Present Regionally restricted 2M A Xist/DXPas34 RNA pinpoint signal is detected on the maternal X chromosome in some cells.
Specimen 2N: embryonic day 3.5 (more )
Note: Early morulas: 8-16 cells. Double labeled: red - DXPas34; green - Xist (assay MGI:7281465).
Structure Level Pattern Image Note
TS4: compacted morula Present Regionally restricted 2N Most cells contain a Xist domain corresponding to the paternal X with no sign of DXPas34 RNA within such domains and a proportion of these cells also contain a Xist/DXPas34 RNA pinpoint signal.
Specimen 2O: embryonic day 4.0 (more )
Note: Blastocyst stage embryo. Double labeled: red - DXPas34; green - Xist (assay MGI:7281465).
Structure Level Pattern Image Note
TS5: inner cell mass Present Regionally restricted 2O A small proportion of cells contain no Xist RNA domain, and either one or two Xist/DXPas34 RNA pinpoint signals.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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