Acan RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:227060 Fujikawa K, et al., An in situ hybridization study of perlecan, DMP1, and MEPE in developing condylar cartilage of the fetal mouse mandible and limb bud cartilage. Eur J Histochem. 2015;59(3):2553
Assay type:	RT-PCR
MGI Accession ID:	MGI:7280933
Gene symbol:	Acan
Gene name:	aggrecan

Probe:	Aggrecan-pG, Aggrecan-pH
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to beta-actin.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	105 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
E14.0	E14.5 (a)	TS22: mandible condylar condensation	Trace (b)	Not Specified; total RNA	ICR		Not Specified
E15.0	E15.5 (a)	TS23: mandible condylar process	Present (c)	Not Specified; total RNA	ICR		Not Specified
E16.0	E16.5 (a)	TS24: mandible condylar process	Very strong (d)	Not Specified; total RNA	ICR		Not Specified

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Authors report expression barely evident.
(c) Expression levels increased modestly compared to E14.
(d) Expression levels markedly upregulated compared to E15..

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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