Gene Expression Data
Assay Details
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Assay
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Reference:
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J:181888
Ashe A, et al., Mutations in mouse Ift144 model the craniofacial, limb and rib defects in skeletal ciliopathies. Hum Mol Genet. 2012 Apr 15;21(8):1808-23
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7279188
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Gene symbol:
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Gli1
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Gene name:
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GLI-Kruppel family member GLI1
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Probe:
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Gli1 probe52
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
S3G:
embryonic day 11.5
(more  )
Specimen
S3G:
(close  )
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Genetic Background:
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involves: FVB/NJ
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
S3G
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Expression in posterior of limb bud.
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Specimen
S3H:
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Wdr19twto/Wdr19twto
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Weak |
Regionally restricted |
S3H
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Expression downregulated, with expression shifted anteriorly, reduced in posterior region.
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Specimen
S3I:
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Wdr19dmhd/Wdr19twto
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Weak |
Regionally restricted |
S3I
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Expression downregulated, with expression shifted anteriorly, reduced in posterior region.
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Specimen
S3J:
embryonic day 11.5
(more )
Specimen
S3J:
(close )
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Genetic Background:
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involves: FVB/NJ
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Present |
Regionally restricted |
S3J
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Expression in posterior of limb bud.
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Specimen
S3K:
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Wdr19twto/Wdr19twto
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Present |
Regionally restricted |
S3K
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Expression expanded into anterior of limb, accompanied by decreased posterior expression.
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Specimen
S3L:
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Wdr19dmhd/Wdr19twto
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Present |
Regionally restricted |
S3L
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Expression expanded into anterior of limb, accompanied by decreased posterior expression.
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Analysis Tools
