Gene Expression Data
Assay Details
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Assay
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Reference:
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J:313342
Nichols DH, et al., Interaction with ectopic cochlear crista sensory epithelium disrupts basal cochlear sensory epithelium development in Lmx1a mutant mice. Cell Tissue Res. 2020 Jun;380(3):435-448
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7279128
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Gene symbol:
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Bmp4
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Gene name:
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bone morphogenetic protein 4
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Probe:
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Bmp4 cDNA5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1a:
embryonic day 11.0
(more  )
Specimen
1a:
(close  )
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Genetic Background:
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involves: 129 * C3H/HeJ * C57BL/6 * CF1
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: inner ear |
Present |
Regionally restricted |
1a
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Expressed in posterior crista and cochlea. Not expressed in utriculo-saccular constriction.
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Specimen
1a':
(close )
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Genetic Background:
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involves: 129 * C3H/HeJ * C57BL/6 * CF1
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Mutant Allele(s):
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Lmx1adr-6J/Lmx1adr-6J
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: inner ear |
Strong |
Regionally restricted |
1a'
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Expressed in posterior crista, cochlea and utriculo-saccular constriction. Expressions were strong at their cores, their edges appeared blurred. An extension of the expression formed a bridge to the posterior wall of the cochlear duct.
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Specimen
1b:
embryonic day 13.5
(more )
Specimen
1b:
(close )
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Genetic Background:
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involves: 129 * C3H/HeJ * C57BL/6 * CF1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: inner ear |
Present |
Regionally restricted |
1b
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Expressed in the utriculo-saccular constriction. Expressed in an unbroken band immediately abneural to the cochlear sensory epithelium.
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| TS21: cochlear duct |
Present |
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1b
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Specimen
1b':
(close )
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Genetic Background:
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involves: 129 * C3H/HeJ * C57BL/6 * CF1
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Mutant Allele(s):
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Lmx1adr-6J/Lmx1adr-6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: inner ear |
Present |
Regionally restricted |
1b'
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Expression extend across the site that would form the utriculosaccular constriction and down the posterior wall of the basal cochlear duct. An unbroken band was present apically, but it weakened basally.
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| TS21: cochlear duct |
Present |
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1b'
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Specimen
1c:
embryonic day 13.5
(more )
Note: Embedded in epoxy resin.
Specimen
1c:
(close )
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Genetic Background:
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involves: 129 * C3H/HeJ * C57BL/6 * CF1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embedded in epoxy resin.
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Specimen
1c':
(close )
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Genetic Background:
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involves: 129 * C3H/HeJ * C57BL/6 * CF1
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Mutant Allele(s):
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Lmx1adr-6J/Lmx1adr-6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embedded in epoxy resin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: semicircular canal |
Present |
Regionally restricted |
1c'
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Expressed in the cochlear crista, located in the basal duct's outer spiral sulcus, immediately adjacent to the cochlear sensory epithelium.
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| TS21: cochlear duct epithelium |
Present |
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1c'
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Specimen
7a:
(close )
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Genetic Background:
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involves: 129 * C3H/HeJ * C57BL/6 * CF1
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Mutant Allele(s):
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Lmx1adr-6J/Lmx1adr-6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Flat mount.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cochlear duct epithelium |
Present |
Patchy |
7a
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Expressed in patches that tapered to extinction as the cochlear sensory epithelium approached its junction with the saccular macula.
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Specimen
7b:
(close )
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Genetic Background:
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involves: 129 * C3H/HeJ * C57BL/6 * CF1
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Mutant Allele(s):
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Lmx1adr-6J/Lmx1adr-6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Flat mount.
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Specimen
7c:
(close )
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Genetic Background:
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involves: 129 * C3H/HeJ * C57BL/6 * CF1
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Mutant Allele(s):
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Lmx1adr-6J/Lmx1adr-6J
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Age:
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postnatal day 4.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Flat mount.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlear duct epithelium |
Present |
Patchy |
7c
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Gaps were variable in length and could include much of the basal cochlear sensory epithelium.
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